Overview
- External ontology
- UTEX:dasycladales-seawater-medium (source link unavailable) — Dasycladales Seawater Medium
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- UTEX prepares Dasycladales Seawater Medium from 30 ppt Pasteurized Seawater, Soil+Seawater Medium, DAS Macro Solution, Modified P-IV chelated Micronutrient Solution, and DAS Vitamin Cocktail. Unresolved source-volume conflict: both HTML and the linked PDF say "For 3 L Total", yet direct preparation of 3 L seawater followed by 121.5 mL of supplements. The seawater fraction is unquantified; four supplement doses explicitly use starting-seawater volume, without substituting an assumed 3.1215 L final volume. Literal batch inputs remain 3 L seawater, 50 mL Soil+Seawater Medium, 40 mL DAS Macro Solution, 30 mL Modified P-IV stock and 1.5 mL vitamin cocktail. Stock compositions are independent of this receiving-volume conflict; no final DAS molarity is recalculated.
Composition
flowchart LR
CultureMech_000161["dasycladales_seawater_medium"]
ing0_Pasteurized_Seawater["Pasteurized Seawater"]
CultureMech_000161 --> ing0_Pasteurized_Seawater
ing1_Soil_Seawater_Medium["Soil+Seawater Medium"]
CultureMech_000161 --> ing1_Soil_Seawater_Medium
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Pasteurized Seawater |
ENVO:00002149
sea water
|
UTEX lists 3 L for the recipe labelled 3 L total. The directions start with 3 L seawater before supplement addition, so the final-volume basis remains unresolved. This literal input is not asserted as a per-final-liter concentration. | |
| 2 | Soil+Seawater Medium | 16.66666666666666666666666667 ML_PER_L (per liter of starting Pasteurized Seawater before supplement addition) | Add 50 mL to the initial 3 L of Pasteurized Seawater. The structured dose is per liter of starting seawater, not per liter of final DAS medium. |
Preparation
- Prepare 3 L of 30 ppt Pasteurized Seawater.
- Add sterile Soil+Seawater Medium, DAS Macro Solution, Modified P-IV chelated Micronutrient Solution, and DAS Vitamin Cocktail to the flask in the listed order.
- Store at refrigerator temperature.
Solutions
DAS Macro Solution
Dose: 13.33333333333333333333333333 ML_PER_L (per liter of starting Pasteurized Seawater before supplement addition)
Add 40 mL to the initial 3 L of Pasteurized Seawater. The structured dose is per liter of starting seawater, not per liter of final DAS medium.
NaNO3 0.70 M stock
Dose: 25 PERCENT_V_V
| Ingredient | Concentration | Notes |
|---|---|---|
| Sodium nitrate | 0.70 MOLAR | Source-explicit component-stock strength; no final DAS concentration inferred. |
Na2HPO4 x 7 H2O 0.02 M stock
Dose: 75 PERCENT_V_V
| Ingredient | Concentration | Notes |
|---|---|---|
| Disodium hydrogen phosphate heptahydrate | 0.02 MOLAR | Exact heptahydrate supplied as Sigma S9390. The former CHEBI:34683 mapping describes the anhydrous salt; exact hydrate ontology grounding remains unresolved. The packaged MIM stock-label answer is not treated as proof of hydrate equivalence. |
Modified P-IV chelated Micronutrient Solution
Dose: 10 ML_PER_L (per liter of starting Pasteurized Seawater before supplement addition)
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | UTEX starts the Modified P-IV recipe with approximately 900 mL dH2O and brings the final volume to 1 L. The stated total is solution volume; the exact final quantity of pure water is not specified. | |
| Na2EDTA x 2 H2O | 0.2 G_PER_L | The source amount is 0.2 g/L. Printed molarity annotations are retained as source assertions only; no receiving-medium molarity is independently verified from them in this review. |
| HEEDTA | 0.03 G_PER_L | The source amount is 0.03 g/L. Printed molarity annotations are retained as source assertions only; no receiving-medium molarity is independently verified from them in this review. |
| FeCl3 x 6 H2O | 0.1 G_PER_L | The source amount is 0.1 g/L. Printed molarity annotations are retained as source assertions only; no receiving-medium molarity is independently verified from them in this review. |
| MnCl2 x 4 H2O | 0.04 G_PER_L | The source amount is 0.04 g/L. Printed molarity annotations are retained as source assertions only; no receiving-medium molarity is independently verified from them in this review. |
| ZnCl2 | 0.005 G_PER_L | The source amount is 0.005 g/L. Printed molarity annotations are retained as source assertions only; no receiving-medium molarity is independently verified from them in this review. |
| CoCl2 x 6 H2O | 0.002 G_PER_L | The source amount is 0.002 g/L. Printed molarity annotations are retained as source assertions only; no receiving-medium molarity is independently verified from them in this review. |
| Na2MoO4 x 2 H2O | 0.004 G_PER_L | The source amount is 0.004 g/L. Printed molarity annotations are retained as source assertions only; no receiving-medium molarity is independently verified from them in this review. |
Add 30 mL to the initial 3 L of Pasteurized Seawater. The structured dose is per liter of starting seawater, not per liter of final DAS medium.
DAS Vitamin Cocktail
Dose: 0.5 ML_PER_L (per liter of starting Pasteurized Seawater before supplement addition)
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | UTEX starts the DAS Vitamin Cocktail with approximately 85 mL dH2O and brings the final volume to 100 mL. The stated total is solution volume; the exact final quantity of pure water is not specified. | |
| Tricine | 9 G_PER_L | The source amount is 0.9 g/100 mL. Printed molarity annotations are retained as source assertions only; no receiving-medium molarity is independently verified from them in this review. |
| Thiamine hydrochloride | 0.67 G_PER_L | The source amount is 0.067 g/100 mL. Printed molarity annotations are retained as source assertions only; no receiving-medium molarity is independently verified from them in this review. |
| Biotin | 0.01 G_PER_L | The source amount is 0.001 g/100 mL. Printed molarity annotations are retained as source assertions only; no receiving-medium molarity is independently verified from them in this review. |
| Vitamin B12 | 0.008 G_PER_L | The B12 table row says add after autoclaving, while the directions specify filtration without an autoclave step. The sterilization sequence remains unresolved. |
| Calcium D-(+)-pantothenate | 0.02 G_PER_L | The source amount is 0.002 g/100 mL. Printed molarity annotations are retained as source assertions only; no receiving-medium molarity is independently verified from them in this review. |
| PABA | 0.04 G_PER_L | The source amount is 0.004 g/100 mL. Printed molarity annotations are retained as source assertions only; no receiving-medium molarity is independently verified from them in this review. |
Add 1.5 mL to the initial 3 L of Pasteurized Seawater. The structured dose is per liter of starting seawater, not per liter of final DAS medium.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | utex-import | Imported from UTEX Culture Collection | Source ID: dasycladales-seawater-medium, URL: https://utex.org/products/dasycladales-seawater-medium |
| 2026-03-13T06:18:53.352873Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000161 |
| 2026-03-17T19:45:04.294707+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Dasycladales Seawater Medium" to "dasycladales_seawater_medium" for consistent matching |
| 2026-05-16T09:02:55.969194+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1 |
| 2026-05-16T09:11:01.166907+00:00 | migrate_preparation_steps.py | MIGRATED_PREPARATION_STEPS | instruction->action+description for 9 step(s) |
| 2026-08-30T00:58:30.756640+00:00 | repair_utex_ingredients.py | REPAIRED_UTEX_COMPOSITION | Restored 5 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779. |
| 2026-09-06T00:00:00-07:00 | repair_utex_dasycladales_pea_score35.py | RESOLVED_UTEX_DASYCLADALES_PEA_SCORE35 | UTEX prepares Dasycladales Seawater Medium from 30 ppt Pasteurized Seawater, Soil+Seawater Medium, DAS Macro Solution, Modified P-IV chelated Micronutrient Solution, and DAS Vitamin Cocktail. |
| 2026-10-10T08:16:36.702755+00:00 | codex | CURATED_UTEX_J_DAS_RECEIVING_MEDIA_EVIDENCE | Evidence-backed amount and stock-boundary review; source conflicts remain explicit. No taxa-media association added. |