Overview
- External ontology
- mediadive.medium:1399 (source link unavailable) — HALOPHILIC MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1399.pdf
Composition
flowchart LR
CultureMech_000252["halophilic_medium"]
ing0_Proteose_peptone_no__3["Proteose peptone no. 3"]
CultureMech_000252 --> ing0_Proteose_peptone_no__3
ing1_Yeast_extract["Yeast extract"]
CultureMech_000252 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_000252 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Agar["Agar"]
CultureMech_000252 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_NaCl["NaCl"]
CultureMech_000252 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000252 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000252 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000252 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_KCl["KCl"]
CultureMech_000252 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_NaBr["NaBr"]
CultureMech_000252 --> ing9_NaBr
CHEBI_63004["CHEBI:63004"]
ing9_NaBr -.-> CHEBI_63004
ing10_NaHCO3["NaHCO3"]
CultureMech_000252 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_000252 --> ing11_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing11_FeCl3_x_6_H2O -.-> CHEBI_86254
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Proteose peptone no. 3 | 5 G_PER_L | ||
| 2 | Yeast extract | 5 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
234 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
42 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
60 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
KCl
Cl.K 74.551 g/mol
|
6 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 10 |
NaBr
Br.Na 102.894 g/mol
|
0.7 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 12 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
9 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Preparation
- Make up to 1000.0 ml. Adjust to pH 7.0 and sterilize by autoclaving. The medium may be solidified by adding 20.0 g/l agar.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- DSMZ_1399_HALOPHILIC_MEDIUM (CultureMech:000252)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.311815Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1399 |
| 2026-03-13T06:18:53.785679Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000252 |
| 2026-03-17T17:06:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:04.693978+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOPHILIC MEDIUM" to "halophilic_medium" for consistent matching |
| 2026-05-16T09:02:57.171288+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-06-05T07:40:14.409593+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.141111+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:20.475634+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8555616f3a1395a643ff430a43a1015794171649870741c46647e8de79ed1076 |