halophilic_medium

CultureMech:000252 archaea COMPLEX SOLID_AGAR
Original name: HALOPHILIC MEDIUM

Overview

External ontology
mediadive.medium:1399 (source link unavailable) — HALOPHILIC MEDIUM
Applications
  • Microbial cultivation
pH
7.0
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1399.pdf

Composition

flowchart LR
    CultureMech_000252["halophilic_medium"]
    ing0_Proteose_peptone_no__3["Proteose peptone no. 3"]
    CultureMech_000252 --> ing0_Proteose_peptone_no__3
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000252 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_000252 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Agar["Agar"]
    CultureMech_000252 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Distilled_water["Distilled water"]
    CultureMech_000252 --> ing4_Distilled_water

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Proteose peptone no. 3 5 G_PER_L Source material: Proteose peptone no. 3 (Difco).
2 Yeast extract 5 G_PER_L
3 Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17234
glucose
4 Agar 20 G_PER_L CHEBI:2509
agar
 (for solid medium)
5 Distilled water 0.333 L mediadive.compound:4 (source link unavailable)
Distilled water

Preparation

  1. Make up to 1000.0 ml. Adjust to pH 7.0 and sterilize by autoclaving. The medium may be solidified by adding 20.0 g/l agar.

Solutions

Total Salts

Dose: 0.666 L

Stock composition
IngredientConcentrationNotes
NaCl 234 G_PER_L
MgCl2 x 6 H2O 42 G_PER_L
MgSO4 x 7 H2O 60 G_PER_L
CaCl2 x 2 H2O 1 G_PER_L
KCl 6 G_PER_L
NaBr 0.7 G_PER_L
NaHCO3 0.2 G_PER_L
FeCl3 x 6 H2O Source dose: 9 drops of FeCl3 x 6 H2O solution (source attribute: 0,5 %) per prepared Total Salts stock batch. Percentage basis and drop volume are unspecified; no g/L, molarity, or mL conversion is asserted.
Distilled water 1 L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.311815Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1399
2026-03-13T06:18:53.785679Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000252
2026-03-17T17:06:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:04.693978+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOPHILIC MEDIUM" to "halophilic_medium" for consistent matching
2026-05-16T09:02:57.171288+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS category->lowercase=1
2026-06-05T07:40:14.409593+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.141111+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T10:22:32.742479+00:00 codex RESTORED_DSMZ1399_STOCK_BOUNDARY_AND_DROP_DOSE Exact medium/stock source identity and original PDF checked. Iron drop dose/percent attribute retained without conversion; percentage basis and drop volume remain unknown.