Overview
- External ontology
- mediadive.medium:458 (source link unavailable) — MEDIUM FOR BACILLUS STEAROTHERMOPHILUS
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'medium_for_bacillus_stearothermophilus_phenol_degrading', 'source': 'KOMODO', 'source_id': 'komodo.medium:458', 'original_category': 'bacterial'}
- pH
- 7.4
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium458.pdf
Composition
flowchart LR
CultureMech_000264["medium_for_bacillus_stearothermophilus"]
ing0_K2HPO4["K2HPO4"]
CultureMech_000264 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_NH4Cl["NH4Cl"]
CultureMech_000264 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000264 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_Yeast_extract["Yeast extract"]
CultureMech_000264 --> ing3_Yeast_extract
ing4_Casamino_acids["Casamino acids"]
CultureMech_000264 --> ing4_Casamino_acids
ing5_Phenol["Phenol"]
CultureMech_000264 --> ing5_Phenol
CHEBI_15882["CHEBI:15882"]
ing5_Phenol -.-> CHEBI_15882
ing6_Na2_EDTA["Na2-EDTA"]
CultureMech_000264 --> ing6_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing6_Na2_EDTA -.-> CHEBI_64734
ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000264 --> ing7_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000264 --> ing8_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000264 --> ing9_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
ing10_H3BO3["H3BO3"]
CultureMech_000264 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000264 --> ing11_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000264 --> ing12_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000264 --> ing13_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000264 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.02 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 | Yeast extract | 0.2 G_PER_L | ||
| 5 | Casamino acids | 0.1 G_PER_L | ||
| 6 |
Phenol
C6H6O 94.113 g/mol
|
100 G_PER_L |
CHEBI:15882
phenol
|
|
| 7 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 8 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 9 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 10 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 12 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 13 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 14 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- pH 7.4
- Autoclave for 15 min. Add 1 ml of sterile SL-4 (see medium 14) and 100 ml of filter-sterilized phenol-solution (4.7g/l).
- First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- medium_for_bacillus_stearothermophilus (CultureMech:000264)
- medium_for_bacillus_stearothermophilus_phenol_degrading (CultureMech:005463)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.194357Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 458 |
| 2026-01-28T19:48:44.716944Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:53.913922Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000264 |
| 2026-03-17T17:06:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:04.833307+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM FOR BACILLUS STEAROTHERMOPHILUS" to "medium_for_bacillus_stearothermophilus" for consistent matching |
| 2026-05-16T09:02:57.362992+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-06-05T07:40:14.639546+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:27.125533+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:42.216143+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:43.031945+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: medium_for_bacillus_stearothermophilus, medium_for_bacillus_stearothermophilus_phenol_degrading |
| 2026-08-06T03:05:43.031997+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint a752f74322fc7de9680d3643b7eb1f83128af53b5c727b40715cfa0034ff9c7c |