medium_for_bacillus_stearothermophilus

CultureMech:000264 bacterial COMPLEX LIQUID
Original name: MEDIUM FOR BACILLUS STEAROTHERMOPHILUS

Overview

External ontology
mediadive.medium:458 (source link unavailable) — MEDIUM FOR BACILLUS STEAROTHERMOPHILUS
Applications
  • Microbial cultivation
pH
7.4
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium458.pdf

Composition

flowchart LR
    CultureMech_000264["medium_for_bacillus_stearothermophilus"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_000264 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_NH4Cl["NH4Cl"]
    CultureMech_000264 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000264 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_000264 --> ing3_Yeast_extract
    ing4_Casamino_acids["Casamino acids"]
    CultureMech_000264 --> ing4_Casamino_acids
    ing5_Phenol["Phenol"]
    CultureMech_000264 --> ing5_Phenol
    CHEBI_15882["CHEBI:15882"]
    ing5_Phenol -.-> CHEBI_15882
    ing6_Na2_EDTA["Na2-EDTA"]
    CultureMech_000264 --> ing6_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing6_Na2_EDTA -.-> CHEBI_64734
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000264 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000264 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000264 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_H3BO3["H3BO3"]
    CultureMech_000264 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000264 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000264 --> ing12_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000264 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000264 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.02 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 Yeast extract 0.2 G_PER_L
5 Casamino acids 0.1 G_PER_L
6 Phenol
C6H6O 94.113 g/mol
100 G_PER_L CHEBI:15882
phenol
7 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.5 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
10 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
11 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. pH 7.4
  2. Autoclave for 15 min. Add 1 ml of sterile SL-4 (see medium 14) and 100 ml of filter-sterilized phenol-solution (4.7g/l).
  3. First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.194357Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 458
2026-01-28T19:48:44.716944Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:53.913922Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000264
2026-03-17T17:06:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:04.833307+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM FOR BACILLUS STEAROTHERMOPHILUS" to "medium_for_bacillus_stearothermophilus" for consistent matching
2026-05-16T09:02:57.362992+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS category->lowercase=1
2026-06-05T07:40:14.639546+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.125533+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:46:42.216143+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.