Overview
- External ontology
- mediadive.medium:470 (source link unavailable) — PSEUDOMONAS HALOPHILA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium470.pdf
Composition
flowchart LR
CultureMech_000265["pseudomonas_halophila_medium"]
ing0_NaCl["NaCl"]
CultureMech_000265 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000265 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_NH4Cl["NH4Cl"]
CultureMech_000265 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Glycerol["Glycerol"]
CultureMech_000265 --> ing3_Glycerol
CHEBI_17754["CHEBI:17754"]
ing3_Glycerol -.-> CHEBI_17754
ing4_KH2PO4["KH2PO4"]
CultureMech_000265 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_HCl["HCl"]
CultureMech_000265 --> ing5_HCl
CHEBI_17883["CHEBI:17883"]
ing5_HCl -.-> CHEBI_17883
ing6_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_000265 --> ing6_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing6_FeCl2_x_4_H2O -.-> CHEBI_86249
ing7_ZnCl2["ZnCl2"]
CultureMech_000265 --> ing7_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing7_ZnCl2 -.-> CHEBI_49976
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000265 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
ing9_H3BO3["H3BO3"]
CultureMech_000265 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000265 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000265 --> ing11_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000265 --> ing12_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000265 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing14_Biotin["Biotin"]
CultureMech_000265 --> ing14_Biotin
CHEBI_15956["CHEBI:15956"]
ing14_Biotin -.-> CHEBI_15956
ing15_Folic_acid["Folic acid"]
CultureMech_000265 --> ing15_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing15_Folic_acid -.-> CHEBI_27470
ing16_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000265 --> ing16_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing16_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing17_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_000265 --> ing17_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing17_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing18_Riboflavin["Riboflavin"]
CultureMech_000265 --> ing18_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing18_Riboflavin -.-> CHEBI_17015
ing19_Nicotinic_acid["Nicotinic acid"]
CultureMech_000265 --> ing19_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing19_Nicotinic_acid -.-> CHEBI_15940
ing20_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_000265 --> ing20_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing20_Calcium_pantothenate -.-> CHEBI_31345
ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_000265 --> ing21_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
ing22_Vitamin_B12["Vitamin B12"]
CultureMech_000265 --> ing22_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing22_Vitamin_B12 -.-> CHEBI_176843
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
51.9423 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
43.7292 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.10988 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
Glycerol
C3H8O3 92.094 g/mol
|
5.54939 G_PER_L |
CHEBI:17754
glycerol
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
10 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 7 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 8 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 13 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 15 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 16 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 17 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 18 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 19 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 20 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 21 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 22 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.001 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 23 | Vitamin B12 | 1e-05 G_PER_L |
CHEBI:176843
vitamin B12
|
Preparation
- Vitamin solution is filter-sterilized and added after autoclaving. Solution A and B are autoclaved separately and combined after cooling.
- Adjust pH to 7.0 with 6 M NaOH or HCl.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- DSMZ_470_PSEUDOMONAS_HALOPHILA_MEDIUM (CultureMech:000265)
- JCM_J433_PSEUDOMONAS_HALOPHILA_MEDIUM (CultureMech:000297)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.276956Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 470 |
| 2026-03-13T06:18:53.923701Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000265 |
| 2026-03-17T17:06:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:04.844274+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PSEUDOMONAS HALOPHILA MEDIUM" to "pseudomonas_halophila_medium" for consistent matching |
| 2026-05-16T09:02:57.775564+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-06-05T07:40:15.072560+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.373021+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:34.659084+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: DSMZ_470_PSEUDOMONAS_HALOPHILA_MEDIUM, JCM_J433_PSEUDOMONAS_HALOPHILA_MEDIUM |
| 2026-08-06T03:04:34.659122+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 4d618a090ffc54dd977683246f0fcb6e763a30579519ce87dbb6c2fa7209248d |