pseudomonas_halophila_medium

CultureMech:000297 bacterial DEFINED LIQUID
Original name: PSEUDOMONAS HALOPHILA MEDIUM

Overview

External ontology
mediadive.medium:J433 (source link unavailable) — PSEUDOMONAS HALOPHILA MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=433

Composition

flowchart LR
    CultureMech_000297["pseudomonas_halophila_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000297 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000297 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NH4Cl["NH4Cl"]
    CultureMech_000297 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_Glycerol["Glycerol"]
    CultureMech_000297 --> ing3_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing3_Glycerol -.-> CHEBI_17754
    ing4_KH2PO4["KH2PO4"]
    CultureMech_000297 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_HCl["HCl"]
    CultureMech_000297 --> ing5_HCl
    CHEBI_17883["CHEBI:17883"]
    ing5_HCl -.-> CHEBI_17883
    ing6_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000297 --> ing6_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing6_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing7_ZnCl2["ZnCl2"]
    CultureMech_000297 --> ing7_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing7_ZnCl2 -.-> CHEBI_49976
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000297 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_H3BO3["H3BO3"]
    CultureMech_000297 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000297 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000297 --> ing11_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000297 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000297 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_Biotin["Biotin"]
    CultureMech_000297 --> ing14_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing14_Biotin -.-> CHEBI_15956
    ing15_Folic_acid["Folic acid"]
    CultureMech_000297 --> ing15_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing15_Folic_acid -.-> CHEBI_27470
    ing16_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000297 --> ing16_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing16_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing17_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_000297 --> ing17_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing17_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing18_Riboflavin["Riboflavin"]
    CultureMech_000297 --> ing18_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing18_Riboflavin -.-> CHEBI_17015
    ing19_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000297 --> ing19_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing19_Nicotinic_acid -.-> CHEBI_15940
    ing20_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000297 --> ing20_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing20_Calcium_pantothenate -.-> CHEBI_31345
    ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000297 --> ing21_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing22_Vitamin_B12["Vitamin B12"]
    CultureMech_000297 --> ing22_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing22_Vitamin_B12 -.-> CHEBI_176843

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
51.9423 G_PER_L CHEBI:26710
sodium chloride
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
43.7292 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 NH4Cl
Cl.H4N 53.492 g/mol
1.10988 G_PER_L CHEBI:31206
ammonium chloride
4 Glycerol
C3H8O3 92.094 g/mol
5.54939 G_PER_L CHEBI:17754
glycerol
5 KH2PO4
H2O4P.K 136.084 g/mol
10 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
7 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
8 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
9 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
10 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
14 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
15 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
16 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
17 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
18 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.005 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
19 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
20 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
21 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
22 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.001 G_PER_L CHEBI:30753
4-aminobenzoic acid
23 Vitamin B12 1e-05 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.
  2. Autoclave Solutions A and B separately and combine after cooling. Filter-sterilize vitamin solution and add to the autoclaved medium.
  3. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.605310Z mediadive-import Imported from MediaDive Source: JCM, ID: J433
2026-03-13T06:18:54.266051Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000297
2026-03-17T17:06:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.223539+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PSEUDOMONAS HALOPHILA MEDIUM" to "pseudomonas_halophila_medium" for consistent matching
2026-05-16T09:02:57.190454+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS category->lowercase=1
2026-06-05T07:40:14.452621+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.147852+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.