s_w_ca

CultureMech:000308 bacterial COMPLEX LIQUID
Original name: S/W + Ca

Overview

External ontology
mediadive.medium:C101 (source link unavailable) — S/W + Ca
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 8.0}
Notes
Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_SW_Ca.pdf

Composition

flowchart LR
    CultureMech_000308["s_w_ca"]
    ing0_Soil["Soil"]
    CultureMech_000308 --> ing0_Soil
    ing1_CaCO3["CaCO3"]
    CultureMech_000308 --> ing1_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing1_CaCO3 -.-> CHEBI_3311

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 Soil variable VARIABLE
2 CaCO3
CO3.Ca 100.086 g/mol
0.01 G_PER_L CHEBI:3311
calcium carbonate

Preparation

  1. Put a layer about 1 cm deep of air-dried, sieved good calcareous garden loam into a test tube or jar. (The use of mud from rivers or ponds is rarely satisfactory.) Carefully add deionised water to a depth of 7 to 10 cm, plug or cover, and steam for one hour or autoclave for 15 minutes at 15 psi (longer for larger vessels) on each of 2 consecutive days; further sterilisation is not needed. Allow to stand for a further day before inoculating, when the pH should be between 7.0 - 8.0.
  2. S/W is prepared as above, but approximately 0.01 g calcium carbonate is placed into the base of the test tube before adding the soil and water.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:19.808777Z mediadive-import Imported from MediaDive Source: CCAP, ID: C101
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Soil
2026-03-13T06:18:54.343516Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000308
2026-03-17T06:30:31.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.308726+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "S/W + Ca" to "s_w_ca" for consistent matching
2026-05-16T09:04:31.109287+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-05-20T06:02:04.599474+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-8' -> {'min': 7.0, 'max': 8.0}
2026-06-05T07:41:44.971014+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:55.047786+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint f6a0f34f8f8aa9d81fdd6312a805820df0b3fda9cb2cfc8e9aafc0ad9934bdf7