Overview
- External ontology
- mediadive.medium:C101 (source link unavailable) — S/W + Ca
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 8.0}
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_SW_Ca.pdf Composition verified against https://www.ccap.ac.uk/wp-content/uploads/MR_SW_Ca.pdf on 2026-08-25.
Composition
flowchart LR
CultureMech_000308["s_w_ca"]
ing0_Calcium_carbonate["Calcium carbonate"]
CultureMech_000308 --> ing0_Calcium_carbonate
CHEBI_3311["CHEBI:3311"]
ing0_Calcium_carbonate -.-> CHEBI_3311
ing1_Air_dried_sieved_calcareous_soil["Air-dried sieved calcareous soil"]
CultureMech_000308 --> ing1_Air_dried_sieved_calcareous_soil
ing2_Deionized_water["Deionized water"]
CultureMech_000308 --> ing2_Deionized_water
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Calcium carbonate
CO3.Ca 100.086 g/mol
|
0.01 g per vessel VARIABLE |
CHEBI:3311
calcium carbonate
|
Place in the base of each vessel before adding soil and water. |
| 2 | Air-dried sieved calcareous soil | variable VARIABLE | Add a layer approximately 1 cm deep to each vessel. | |
| 3 | Deionized water | variable VARIABLE | Add carefully to a depth of 7-10 cm above the soil layer. |
Preparation
- Place approximately 0.01 g calcium carbonate in the base of each vessel.
- Place a 1 cm layer of air-dried, sieved calcareous soil in each vessel.
- Optionally add one grain for organisms that benefit from it.
- Add deionized water carefully to a depth of 7-10 cm, then plug or cover.
- Autoclave at 15 psi for 15 minutes on each of two consecutive days.
- Let stand for at least one further day before inoculation.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.808777Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C101 |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Soil |
| 2026-03-13T06:18:54.343516Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000308 |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.308726+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "S/W + Ca" to "s_w_ca" for consistent matching |
| 2026-05-16T09:04:31.109287+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-05-20T06:02:04.599474+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7-8' -> {'min': 7.0, 'max': 8.0} |
| 2026-06-05T07:41:44.971014+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-25T00:00:00-07:00 | repair_ccap_duplicate_compositions.py | SOURCE_CORRECTED_CCAP_DUPLICATE_RECIPE | Rebuilt sw_ca from the reviewed CCAP PDF. Preserved stock boundaries and per-vessel quantities; ingredient identities follow the MediaIngredientMech SSSOM. |
| 2026-09-10T00:00:00-07:00 | repair_ccap_sw_flags_score20.py | MARKED_CCAP_SW_SPARSE_SCORE20_GRAPH | Marked reviewed CCAP S/W soil-water components as intentionally curated with unmapped bulk material identities. |