bg11 (CultureMech:000324)

Overview

External ontology

mediadive.medium:C17 (source link unavailable) — BG11

Applications

Microbial cultivation

pH

7.1

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_BG11.pdf

Composition

flowchart LR
    CultureMech_000324["bg11"]
    ing0_NaNO3["NaNO3"]
    CultureMech_000324 --> ing0_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing0_NaNO3 -.-> CHEBI_63005
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000324 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000324 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000324 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Citric_acid["Citric acid"]
    CultureMech_000324 --> ing4_Citric_acid
    CHEBI_30769["CHEBI:30769"]
    ing4_Citric_acid -.-> CHEBI_30769
    ing5_Ammonium_ferric_citrate["Ammonium ferric citrate"]
    CultureMech_000324 --> ing5_Ammonium_ferric_citrate
    CHEBI_31604["CHEBI:31604"]
    ing5_Ammonium_ferric_citrate -.-> CHEBI_31604
    ing6_Na2_EDTA["Na2-EDTA"]
    CultureMech_000324 --> ing6_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing6_Na2_EDTA -.-> CHEBI_64734
    ing7_Na2CO3["Na2CO3"]
    CultureMech_000324 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_Agar["Agar"]
    CultureMech_000324 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_H3BO3["H3BO3"]
    CultureMech_000324 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000324 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000324 --> ing11_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000324 --> ing12_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000324 --> ing13_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing14_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_000324 --> ing14_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing14_Co_NO3_2_x_6_H2O -.-> CHEBI_86214

Ingredients (15)

#	Ingredient	Concentration	Ontology	Notes
1	NaNO3 NO3.Na 84.994 g/mol	10 G_PER_L	CHEBI:63005 sodium nitrate
2	K2HPO4 HO4P.2K 174.174 g/mol	10 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
3	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	10 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
4	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	10 G_PER_L	CHEBI:86158 calcium chloride dihydrate
5	Citric acid C6H8O7 192.123 g/mol	10 G_PER_L	CHEBI:30769 citric acid
6	Ammonium ferric citrate	10 G_PER_L	CHEBI:31604 ferric ammonium citrate
7	Na2-EDTA C10H14N2O8.2Na 336.208 g/mol	10 G_PER_L	CHEBI:64734 EDTA disodium salt (anhydrous)
8	Na2CO3 CO3.2Na 105.988 g/mol	25.3 G_PER_L	CHEBI:29377 sodium carbonate
9	Agar	15 G_PER_L	CHEBI:2509 agar	(for solid medium)
10	H3BO3 H3BO3 61.833 g/mol	2.86 G_PER_L	CHEBI:33118 boric acid
11	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	1.81 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate
12	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.22 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate
13	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.39 G_PER_L	CHEBI:75213 sodium molybdate dihydrate
14	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	0.08 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate
15	Co(NO3)2 x 6 H2O Co.6H2O.2NO3 291.031 g/mol	0.05 G_PER_L	CHEBI:86214 cobalt dinitrate hexahydrate

Preparation

Make up to 1 litre with deionized water. Adjust pH to 7.1 with 1M NaOH or HCl. For agar add 15.0 g per litre of Bacteriological Agar (Thermo Scientific™ Oxoid™ Agar No.1). Autoclave at 15 psi for 15 minutes.
Due to precipitation, larger volumes require stocks of citric acid and ammonium ferric citrate to be autoclaved separately in 100ml deionized water or alternatively they can be autoclaved separately in test tubes and added to sterile medium in the airflow cabinet.

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

Preferred term
Synechocystis sp. PCC 6803
Term
Id
NCBITaxon:1148
Label
Synechocystis sp. PCC 6803
Growth metrics
- Growth rate per hour
  0.16
  Doubling time minutes
  258.0
  Evidence
  Reference
  PMID:29577667
  Supports
  SUPPORT
  Snippet
  Optimizing these growth conditions resulted in a so far highest growth rate of 0.16 h-1 (4.3 h doubling time), which is significantly higher than the textbook value of 0.09 h-1 (8 h doubling time).
  Explanation
  Auto-applied from literature query: "bg11"[All Fields] AND doubling time[All Fields]
  Is max attainment
  True
Preferred term
Synechococcus elongatus PCC 11801
Term
Id
NCBITaxon:2219813
Label
Synechococcus elongatus PCC 11801
Growth metrics
- Doubling time minutes
  138.0
  Evidence
  Reference
  PMID:30413737
  Supports
  SUPPORT
  Snippet
  PCC 11801 shows a doubling time of 2.3 h, that is the fastest growth for any cyanobacteria reported so far under ambient CO2 conditions.
  Explanation
  Auto-applied from literature query: targeted: Synechococcus elongatus PCC 11801
  Is max attainment
  True
Preferred term
Synechococcus elongatus PCC 11802
Term
Id
NCBITaxon:2283154
Label
Synechococcus elongatus PCC 11802
Growth metrics
- Doubling time minutes
  168.0
  Evidence
  Reference
  PMID:31932622
  Supports
  SUPPORT
  Snippet
  The new isolate has a doubling time of 2.8 h at 1% CO2, 1000 µmole photons.m-2.s-1 and grows faster under high CO2 and temperature compared to PCC 11801 thus making it an attractive host for outdoor cultivations and eventual applications in the biorefinery.
  Explanation
  Auto-applied from literature query: targeted: Synechococcus elongatus PCC 11802
  Is max attainment
  True

Recipe variants

Name
optimized_bg11_synechocystis_pcc6803
Description
Optimized BG11 growth condition for Synechocystis sp. PCC 6803.
Modifications
- Use Synechocystis sp. PCC 6803 as the target organism.
- Apply optimized growth conditions reported by the source study.
Purpose
Capture optimized BG11 growth-rate evidence under the parent BG11 record.
Evidence
- Reference
  PMID:29577667
  Supports
  SUPPORT
  Snippet
  Optimizing these growth conditions resulted in a so far highest growth rate of 0.16 h-1 (4.3 h doubling time), which is significantly higher than the textbook value of 0.09 h-1 (8 h doubling time).
  Explanation
  Literature evidence supports a PCC 6803 optimized-growth condition variant under the parent BG11 record.
Name
ambient_co2_bg11_synechococcus_pcc11801
Description
Ambient-CO2 BG11 growth condition for Synechococcus elongatus PCC 11801.
Modifications
- Use Synechococcus elongatus PCC 11801 as the target strain.
- Culture under ambient CO2 conditions.
Purpose
Capture strain-specific BG11 growth evidence under ambient CO2.
Evidence
- Reference
  PMID:30413737
  Supports
  SUPPORT
  Snippet
  PCC 11801 shows a doubling time of 2.3 h, that is the fastest growth for any cyanobacteria reported so far under ambient CO2 conditions.
  Explanation
  Literature evidence supports an ambient-CO2 PCC 11801 condition variant under the parent BG11 record.
Name
high_co2_high_light_bg11_synechococcus_pcc11802
Description
High-CO2, high-light BG11 growth condition for Synechococcus elongatus PCC 11802.
Modifications
- Use Synechococcus elongatus PCC 11802 as the target strain.
- Culture at 1% CO2.
- Culture at 1000 umole photons m-2 s-1.
Purpose
Capture strain-specific high-CO2/high-light BG11 growth evidence.
Evidence
- Reference
  PMID:31932622
  Supports
  SUPPORT
  Snippet
  The new isolate has a doubling time of 2.8 h at 1% CO2, 1000 µmole photons.m-2.s-1 and grows faster under high CO2 and temperature compared to PCC 11801 thus making it an attractive host for outdoor cultivations and eventual applications in the biorefinery.
  Explanation
  Literature evidence supports a high-CO2/high-light PCC 11802 condition variant under the parent BG11 record.

Provenance

Merged from 1 source record.

bg11 (normalized source unresolved)

Timestamp	Curator	Action	Notes
2026-01-26T17:53:19.526682Z	mediadive-import	Imported from MediaDive	Source: CCAP, ID: C17
2026-03-13T06:18:54.466219Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000324
2026-03-17T06:30:25.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.445173+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "BG11" to "bg11" for consistent matching
2026-05-04T02:04:34.256015+00:00	literature_review_apply	ADDED_GROWTH_EVIDENCE	organisms_added=1 metrics_added=1 genomes_added=0
2026-05-04T02:38:51.030913+00:00	literature_review_apply	ADDED_GROWTH_EVIDENCE	organisms_added=2 metrics_added=2 genomes_added=0
2026-06-05T07:40:50.800655+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.872276+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T00:58:02.702712+00:00	curate_organism_culture_type.py	SET_ORGANISM_CULTURE_TYPE	Inferred isolate: 3 specific strain(s) named, no community signal (#142)
2026-08-06T03:05:25.052102+00:00	merge_recipes.py	MERGED_RECIPES	merged 1 source recipe(s) on fingerprint f771062f80aa643e47556ee4820cb01e4b669b56231c092bbb9bf3ad8abc4f13