Overview
- External ontology
- mediadive.medium:C17 (source link unavailable) — BG11
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_BG11.pdf
Composition
flowchart LR
CultureMech_000324["bg11"]
ing0_NaNO3["NaNO3"]
CultureMech_000324 --> ing0_NaNO3
CHEBI_63005["CHEBI:63005"]
ing0_NaNO3 -.-> CHEBI_63005
ing1_K2HPO4["K2HPO4"]
CultureMech_000324 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000324 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000324 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_Citric_acid["Citric acid"]
CultureMech_000324 --> ing4_Citric_acid
CHEBI_30769["CHEBI:30769"]
ing4_Citric_acid -.-> CHEBI_30769
ing5_Ammonium_ferric_citrate["Ammonium ferric citrate"]
CultureMech_000324 --> ing5_Ammonium_ferric_citrate
CHEBI_31604["CHEBI:31604"]
ing5_Ammonium_ferric_citrate -.-> CHEBI_31604
ing6_Na2_EDTA["Na2-EDTA"]
CultureMech_000324 --> ing6_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing6_Na2_EDTA -.-> CHEBI_64734
ing7_Na2CO3["Na2CO3"]
CultureMech_000324 --> ing7_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing7_Na2CO3 -.-> CHEBI_29377
ing8_Agar["Agar"]
CultureMech_000324 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
ing9_H3BO3["H3BO3"]
CultureMech_000324 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000324 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000324 --> ing11_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000324 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000324 --> ing13_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
ing14_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
CultureMech_000324 --> ing14_Co_NO3_2_x_6_H2O
CHEBI_86214["CHEBI:86214"]
ing14_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaNO3
NO3.Na 84.994 g/mol
|
10 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
10 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
10 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
10 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
Citric acid
C6H8O7 192.123 g/mol
|
10 G_PER_L |
CHEBI:30769
citric acid
|
|
| 6 | Ammonium ferric citrate | 10 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 7 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
10 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 8 |
Na2CO3
CO3.2Na 105.988 g/mol
|
25.3 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
2.86 G_PER_L |
CHEBI:33118
boric acid
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.81 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.39 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 14 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.08 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 15 |
Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.05 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Preparation
- Make up to 1 litre with deionized water. Adjust pH to 7.1 with 1M NaOH or HCl. For agar add 15.0 g per litre of Bacteriological Agar (Thermo Scientific™ Oxoid™ Agar No.1). Autoclave at 15 psi for 15 minutes.
- Due to precipitation, larger volumes require stocks of citric acid and ammonium ferric citrate to be autoclaved separately in 100ml deionized water or alternatively they can be autoclaved separately in test tubes and added to sterile medium in the airflow cabinet.
Target organisms and growth evidence
These are curated observations and conditions for named organisms; they do not imply universal growth suitability.
- Preferred term
- Synechocystis sp. PCC 6803
- Term
- Id
- NCBITaxon:1148
- Label
- Synechocystis sp. PCC 6803
- Growth metrics
- Growth rate per hour
- 0.16
- Doubling time minutes
- 258.0
- Evidence
- Reference
- PMID:29577667
- Supports
- SUPPORT
- Snippet
- Optimizing these growth conditions resulted in a so far highest growth rate of 0.16 h-1 (4.3 h doubling time), which is significantly higher than the textbook value of 0.09 h-1 (8 h doubling time).
- Explanation
- Auto-applied from literature query: "bg11"[All Fields] AND doubling time[All Fields]
- Is max attainment
- True
- Preferred term
- Synechococcus elongatus PCC 11801
- Term
- Id
- NCBITaxon:2219813
- Label
- Synechococcus elongatus PCC 11801
- Growth metrics
- Doubling time minutes
- 138.0
- Evidence
- Reference
- PMID:30413737
- Supports
- SUPPORT
- Snippet
- PCC 11801 shows a doubling time of 2.3 h, that is the fastest growth for any cyanobacteria reported so far under ambient CO2 conditions.
- Explanation
- Auto-applied from literature query: targeted: Synechococcus elongatus PCC 11801
- Is max attainment
- True
- Preferred term
- Synechococcus elongatus PCC 11802
- Term
- Id
- NCBITaxon:2283154
- Label
- Synechococcus elongatus PCC 11802
- Growth metrics
- Doubling time minutes
- 168.0
- Evidence
- Reference
- PMID:31932622
- Supports
- SUPPORT
- Snippet
- The new isolate has a doubling time of 2.8 h at 1% CO2, 1000 µmole photons.m-2.s-1 and grows faster under high CO2 and temperature compared to PCC 11801 thus making it an attractive host for outdoor cultivations and eventual applications in the biorefinery.
- Explanation
- Auto-applied from literature query: targeted: Synechococcus elongatus PCC 11802
- Is max attainment
- True
Recipe variants
- Name
- optimized_bg11_synechocystis_pcc6803
- Description
- Optimized BG11 growth condition for Synechocystis sp. PCC 6803.
- Modifications
- Use Synechocystis sp. PCC 6803 as the target organism.
- Apply optimized growth conditions reported by the source study.
- Purpose
- Capture optimized BG11 growth-rate evidence under the parent BG11 record.
- Evidence
- Reference
- PMID:29577667
- Supports
- SUPPORT
- Snippet
- Optimizing these growth conditions resulted in a so far highest growth rate of 0.16 h-1 (4.3 h doubling time), which is significantly higher than the textbook value of 0.09 h-1 (8 h doubling time).
- Explanation
- Literature evidence supports a PCC 6803 optimized-growth condition variant under the parent BG11 record.
- Name
- ambient_co2_bg11_synechococcus_pcc11801
- Description
- Ambient-CO2 BG11 growth condition for Synechococcus elongatus PCC 11801.
- Modifications
- Use Synechococcus elongatus PCC 11801 as the target strain.
- Culture under ambient CO2 conditions.
- Purpose
- Capture strain-specific BG11 growth evidence under ambient CO2.
- Evidence
- Reference
- PMID:30413737
- Supports
- SUPPORT
- Snippet
- PCC 11801 shows a doubling time of 2.3 h, that is the fastest growth for any cyanobacteria reported so far under ambient CO2 conditions.
- Explanation
- Literature evidence supports an ambient-CO2 PCC 11801 condition variant under the parent BG11 record.
- Name
- high_co2_high_light_bg11_synechococcus_pcc11802
- Description
- High-CO2, high-light BG11 growth condition for Synechococcus elongatus PCC 11802.
- Modifications
- Use Synechococcus elongatus PCC 11802 as the target strain.
- Culture at 1% CO2.
- Culture at 1000 umole photons m-2 s-1.
- Purpose
- Capture strain-specific high-CO2/high-light BG11 growth evidence.
- Evidence
- Reference
- PMID:31932622
- Supports
- SUPPORT
- Snippet
- The new isolate has a doubling time of 2.8 h at 1% CO2, 1000 µmole photons.m-2.s-1 and grows faster under high CO2 and temperature compared to PCC 11801 thus making it an attractive host for outdoor cultivations and eventual applications in the biorefinery.
- Explanation
- Literature evidence supports a high-CO2/high-light PCC 11802 condition variant under the parent BG11 record.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.526682Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C17 |
| 2026-03-13T06:18:54.466219Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000324 |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.445173+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BG11" to "bg11" for consistent matching |
| 2026-05-04T02:04:34.256015+00:00 | literature_review_apply | ADDED_GROWTH_EVIDENCE | organisms_added=1 metrics_added=1 genomes_added=0 |
| 2026-05-04T02:38:51.030913+00:00 | literature_review_apply | ADDED_GROWTH_EVIDENCE | organisms_added=2 metrics_added=2 genomes_added=0 |
| 2026-06-05T07:40:50.800655+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.872276+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T00:58:02.702712+00:00 | curate_organism_culture_type.py | SET_ORGANISM_CULTURE_TYPE | Inferred isolate: 3 specific strain(s) named, no community signal (#142) |