Overview
- External ontology
- mediadive.medium:C39 (source link unavailable) — HSM
- Applications
-
- Microbial cultivation
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_HSM.pdf
Composition
flowchart LR
CultureMech_000348["hsm"]
ing0_Horse_serum["Horse serum"]
CultureMech_000348 --> ing0_Horse_serum
ing1_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_000348 --> ing1_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing1_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing2_KH2PO4["KH2PO4"]
CultureMech_000348 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NaCl["NaCl"]
CultureMech_000348 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_Marmite["Marmite"]
CultureMech_000348 --> ing4_Marmite
ing5_Rice_starch["Rice starch"]
CultureMech_000348 --> ing5_Rice_starch
CHEBI_28017["CHEBI:28017"]
ing5_Rice_starch -.-> CHEBI_28017
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Horse serum | 0.5 G_PER_L | ||
| 2 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
2.65 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.41 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
7.36 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 | Marmite | 10 G_PER_L | ||
| 6 | Rice starch | 250 G_PER_L |
CHEBI:28017
starch
|
Preparation
- Aseptically add stock solutions (2), (3) and (4) in above amounts to each test tube. Incubate at room temperature for 3 days to check sterility prior to use.
- Dispense 8.5 ml aliquots into test tubes. Cap and autoclave at 15 psi for 15 minutes.
- Sterilise by passing through 0.22 µm filter.
- Place dry rice starch into a dry 50 ml bottle, and cap. Place 20 ml deionised water into a separate bottle. Autoclave separately at 15 psi for 15 minutes. When cool, aseptically combine the contents of the two bottles. Mix thoroughly by shaking.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- hsm (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.603970Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C39 |
| 2026-03-13T06:18:54.646515Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000348 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.640222+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HSM" to "hsm" for consistent matching |
| 2026-06-05T07:41:08.789200+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.841527+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:37.906150+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 51802e7e02d59540b9024c92683c2a561db4cd090b0567625ea85fc0701da16a |