hsm

Overview

External ontology

mediadive.medium:C39 (source link unavailable) — HSM

Applications

• Microbial cultivation

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_HSM.pdf

Composition

flowchart LR
    CultureMech_000348["hsm"]
    ing0_Horse_serum["Horse serum"]
    CultureMech_000348 --> ing0_Horse_serum
    ing1_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_000348 --> ing1_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing1_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing2_KH2PO4["KH2PO4"]
    CultureMech_000348 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NaCl["NaCl"]
    CultureMech_000348 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_Marmite["Marmite"]
    CultureMech_000348 --> ing4_Marmite
    ing5_Rice_starch["Rice starch"]
    CultureMech_000348 --> ing5_Rice_starch
    CHEBI_28017["CHEBI:28017"]
    ing5_Rice_starch -.-> CHEBI_28017

Ingredients (6)

Preparation

1. Aseptically add stock solutions (2), (3) and (4) in above amounts to each test tube. Incubate at room temperature for 3 days to check sterility prior to use.
2. Dispense 8.5 ml aliquots into test tubes. Cap and autoclave at 15 psi for 15 minutes.
3. Sterilise by passing through 0.22 µm filter.
4. Place dry rice starch into a dry 50 ml bottle, and cap. Place 20 ml deionised water into a separate bottle. Autoclave separately at 15 psi for 15 minutes. When cool, aseptically combine the contents of the two bottles. Mix thoroughly by shaking.

Target organisms

No target organisms are recorded.

Provenance