Overview
- External ontology
- mediadive.medium:C68 (source link unavailable) — NN
- Applications
-
- Microbial cultivation
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_NN.pdf
Composition
flowchart LR
CultureMech_000380["nn"]
ing0_Agar["Agar"]
CultureMech_000380 --> ing0_Agar
CHEBI_2509["CHEBI:2509"]
ing0_Agar -.-> CHEBI_2509
ing1_NaCl["NaCl"]
CultureMech_000380 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000380 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
CultureMech_000380 --> ing3_CaCl2_x_6_H2O
CHEBI_91243["CHEBI:91243"]
ing3_CaCl2_x_6_H2O -.-> CHEBI_91243
ing4_Na2HPO4["Na2HPO4"]
CultureMech_000380 --> ing4_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing4_Na2HPO4 -.-> CHEBI_34683
ing5_KH2PO4["KH2PO4"]
CultureMech_000380 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
24 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.8 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
|
1.2 G_PER_L |
CHEBI:91243
calcium chloride hexahydrate
|
|
| 5 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
28.4 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
27.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Preparation
- Add stock solutions to deionized water and make up to 1 litre. In suitable vessels thoroughly disperse the agar in cold amoeba saline solution. Autoclave at 15 psi for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- nn (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.711703Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C68 |
| 2026-03-13T06:18:54.866732Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000380 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.876375+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NN" to "nn" for consistent matching |
| 2026-06-05T07:41:40.048540+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.675618+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:51.609380+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a113b19935c2148349eeddc0104dd2f004c5574c7c6eac3da80209710076c4f8 |