nn

CultureMech:000380 bacterial DEFINED SOLID_AGAR
Original name: NN

Overview

External ontology
mediadive.medium:C68 (source link unavailable) — NN
Applications
  • Microbial cultivation
Notes
Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_NN.pdf

Composition

flowchart LR
    CultureMech_000380["nn"]
    ing0_Agar["Agar"]
    CultureMech_000380 --> ing0_Agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Agar -.-> CHEBI_2509
    ing1_NaCl["NaCl"]
    CultureMech_000380 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000380 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
    CultureMech_000380 --> ing3_CaCl2_x_6_H2O
    CHEBI_91243["CHEBI:91243"]
    ing3_CaCl2_x_6_H2O -.-> CHEBI_91243
    ing4_Na2HPO4["Na2HPO4"]
    CultureMech_000380 --> ing4_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing4_Na2HPO4 -.-> CHEBI_34683
    ing5_KH2PO4["KH2PO4"]
    CultureMech_000380 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Agar 15 G_PER_L CHEBI:2509
agar
2 NaCl
Cl.Na 58.443 g/mol
24 G_PER_L CHEBI:26710
sodium chloride
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.8 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
1.2 G_PER_L CHEBI:91243
calcium chloride hexahydrate
5 Na2HPO4
HO4P.2Na 141.958 g/mol
28.4 G_PER_L CHEBI:34683
disodium hydrogenphosphate
6 KH2PO4
H2O4P.K 136.084 g/mol
27.2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate

Preparation

  1. Add stock solutions to deionized water and make up to 1 litre. In suitable vessels thoroughly disperse the agar in cold amoeba saline solution. Autoclave at 15 psi for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.711703Z mediadive-import Imported from MediaDive Source: CCAP, ID: C68
2026-03-13T06:18:54.866732Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000380
2026-03-17T06:30:05.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.876375+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NN" to "nn" for consistent matching
2026-06-05T07:41:40.048540+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.675618+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.