Overview
- External ontology
- mediadive.medium:C73 (source link unavailable) — PC
- Applications
-
- Microbial cultivation
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_PC.pdf
Composition
flowchart LR
CultureMech_000386["pc"]
ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000386 --> ing0_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
ing1_KCl["KCl"]
CultureMech_000386 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_CaCl2["CaCl2"]
CultureMech_000386 --> ing2_CaCl2
CHEBI_3312["CHEBI:3312"]
ing2_CaCl2 -.-> CHEBI_3312
ing3_NaCl["NaCl"]
CultureMech_000386 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaHPO4["CaHPO4"]
CultureMech_000386 --> ing4_CaHPO4
CHEBI_32596["CHEBI:32596"]
ing4_CaHPO4 -.-> CHEBI_32596
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
CaCl2
Ca.2Cl 110.984 g/mol
|
1 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
CaHPO4
Ca.HO4P 136.056 g/mol
|
0.36 G_PER_L |
CHEBI:32596
calcium hydrogenphosphate
|
Preparation
- Using aseptic technique, make up to 1 litre with sterile deionized water. It may be necessary to decant supernatant from precipitate in final medium.
- Alternatively, after autoclaving, it might be necessary to filter through 0.2 micron filter (removes precipitates etc).
- Autoclave at 15 psi for 15 minutes. Dispense any excess stocks into 10 ml aliquots and freeze.
- Autoclave at 15 psi for 15 minutes. Dispense any excess stocks into 10 ml aliquots and freeze.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pc (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.725970Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C73 |
| 2026-03-13T06:18:54.888312Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000386 |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.898328+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PC" to "pc" for consistent matching |
| 2026-06-05T07:41:40.978004+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:52.292104+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c50558b8c109f5da18144867240e434075bc8f58b5bf47b5744775a55c988617 |