rpl_pj

Overview

External ontology

mediadive.medium:C84 (source link unavailable) — RPL:PJ

Applications

• Microbial cultivation

Synonyms

• {'name': 'rpl', 'source': 'MediaDive', 'source_id': 'mediadive.medium:C82', 'original_category': 'bacterial'}

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_RPL_PJ.pdf

Composition

flowchart LR
    CultureMech_000398["rpl_pj"]
    ing0_Rye_grass["Rye grass"]
    CultureMech_000398 --> ing0_Rye_grass
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000398 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000398 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000398 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KCl["KCl"]
    CultureMech_000398 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588

Ingredients (5)

Preparation

1. 3:1 mixture
2. See separate recipes. Autoclave separately. Mix aseptically when cool.
3. Bring PJ (Mail sol. C 76) to the boil then add rye grass. Continue to boil for 5 minutes. Allow to cool then restore volume to 1 litre with deionized water. Filter through GF/C paper and autoclave at 15 psi for 15 minutes.
4. Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

• rpl (normalized source unresolved)
• rpl_pj (normalized source unresolved)