rpl_pj

CultureMech:000398 bacterial COMPLEX LIQUID
Original name: RPL:PJ

Overview

External ontology
mediadive.medium:C84 (source link unavailable) — RPL:PJ
Applications
  • Microbial cultivation
Notes
Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_RPL_PJ.pdf

Composition

flowchart LR
    CultureMech_000398["rpl_pj"]
    ing0_Rye_grass["Rye grass"]
    CultureMech_000398 --> ing0_Rye_grass
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000398 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000398 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000398 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KCl["KCl"]
    CultureMech_000398 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Rye grass 1 G_PER_L
2 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
4.3 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 KCl
Cl.K 74.551 g/mol
1.6 G_PER_L CHEBI:32588
potassium chloride

Preparation

  1. 3:1 mixture
  2. See separate recipes. Autoclave separately. Mix aseptically when cool.
  3. Bring PJ (Mail sol. C 76) to the boil then add rye grass. Continue to boil for 5 minutes. Allow to cool then restore volume to 1 litre with deionized water. Filter through GF/C paper and autoclave at 15 psi for 15 minutes.
  4. Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.756468Z mediadive-import Imported from MediaDive Source: CCAP, ID: C84
2026-03-13T06:18:54.942088Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000398
2026-03-17T06:30:34.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.955218+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RPL:PJ" to "rpl_pj" for consistent matching
2026-06-05T07:41:44.701020+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.093586+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.