Overview
- External ontology
- mediadive.medium:1040 (source link unavailable) — MARIDESULFOVIBRIO MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'for_dsm_17682', 'source': 'KOMODO', 'source_id': 'komodo.medium:1040.1', 'original_category': 'bacterial'}
- {'name': 'sylc_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:1040', 'original_category': 'bacterial'}
- pH range
- {'min': 7.0, 'max': 7.2}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1040.pdf
Composition
flowchart LR
CultureMech_000463["maridesulfovibrio_medium"]
ing0_Sea_Salt["Sea Salt"]
CultureMech_000463 --> ing0_Sea_Salt
ing1_Yeast_extract["Yeast extract"]
CultureMech_000463 --> ing1_Yeast_extract
ing2_Sodium_resazurin["Sodium resazurin"]
CultureMech_000463 --> ing2_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Sodium_resazurin -.-> CHEBI_8806
ing3_Na2CO3["Na2CO3"]
CultureMech_000463 --> ing3_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing3_Na2CO3 -.-> CHEBI_29377
ing4_Na_DL_lactate["Na-DL-lactate"]
CultureMech_000463 --> ing4_Na_DL_lactate
CHEBI_75228["CHEBI:75228"]
ing4_Na_DL_lactate -.-> CHEBI_75228
ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_000463 --> ing5_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing5_Na2S_x_9_H2O -.-> CHEBI_76209
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000463 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_000463 --> ing7_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing7_MnSO4_x_H2O -.-> CHEBI_86364
ing8_NaCl["NaCl"]
CultureMech_000463 --> ing8_NaCl
CHEBI_26710["CHEBI:26710"]
ing8_NaCl -.-> CHEBI_26710
ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000463 --> ing9_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000463 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000463 --> ing11_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000463 --> ing12_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000463 --> ing13_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
ing14_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_000463 --> ing14_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing14_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing15_H3BO3["H3BO3"]
CultureMech_000463 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000463 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_000463 --> ing17__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing17__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing18_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_000463 --> ing18_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing18_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing19_Na2SeO4["Na2SeO4"]
CultureMech_000463 --> ing19_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing19_Na2SeO4 -.-> CHEBI_77775
ing20_Biotin["Biotin"]
CultureMech_000463 --> ing20_Biotin
CHEBI_15956["CHEBI:15956"]
ing20_Biotin -.-> CHEBI_15956
ing21_Folic_acid["Folic acid"]
CultureMech_000463 --> ing21_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing21_Folic_acid -.-> CHEBI_27470
ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000463 --> ing22_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing23_Thiamine_HCl["Thiamine HCl"]
CultureMech_000463 --> ing23_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing23_Thiamine_HCl -.-> CHEBI_49105
ing24_Riboflavin["Riboflavin"]
CultureMech_000463 --> ing24_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing24_Riboflavin -.-> CHEBI_17015
ing25_Nicotinic_acid["Nicotinic acid"]
CultureMech_000463 --> ing25_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing25_Nicotinic_acid -.-> CHEBI_15940
ing26_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_000463 --> ing26_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing26_Calcium_D_____pantothenate -.-> CHEBI_31345
ing27_Vitamin_B12["Vitamin B12"]
CultureMech_000463 --> ing27_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing27_Vitamin_B12 -.-> CHEBI_176843
ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_000463 --> ing28_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
ing29__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_000463 --> ing29__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing29__DL__alpha_Lipoic_acid -.-> CHEBI_16494
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea Salt | 34.9301 G_PER_L | ||
| 2 | Yeast extract | 0.998004 G_PER_L | ||
| 3 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000499002 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 4 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.998004 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 5 |
Na-DL-lactate
C3H5O3.Na 112.06 g/mol
|
2.49501 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 6 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.299401 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
30 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 9 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 10 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1.8 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 13 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.8 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 14 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 15 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.18 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2.8 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 19 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 20 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.1 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 21 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 22 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 23 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 24 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 25 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 26 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 27 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 28 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 29 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 30 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients (except carbonate, lactate, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add lactate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. The vitamin solution should be sterilized by filtration. Adjust pH of the complete medium to 7.0-7.2, if necessary.
- First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- for_dsm_17682 (CultureMech:003597)
- maridesulfovibrio_medium (CultureMech:000463)
- sylc_medium (CultureMech:003598)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.178320Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1040 |
| 2026-02-06T05:10:21.084617Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:18:55.527709Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000463 |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.529193+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MARIDESULFOVIBRIO MEDIUM" to "maridesulfovibrio_medium" for consistent matching |
| 2026-05-20T06:01:25.368409+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2} |
| 2026-06-05T07:41:12.733741+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.069994+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:00.850194+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:34.971442+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: for_dsm_17682, maridesulfovibrio_medium, sylc_medium |
| 2026-08-06T03:05:34.971509+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 74f9294fa8a3ca2d3870b15d95addc5ae345a85b91ec7e6c886a389276ca6f8a |