Overview
- External ontology
- komodo.medium:1040.1 (source link unavailable) — For DSM 17682
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.2}
- Notes
- Source: KOMODO ModelSEED | ID: 1040.1 | DSMZ Medium: 1040 (mediadive.medium:1040) | Aerobic: Yes
Composition
flowchart LR
CultureMech_003597["for_dsm_17682"]
ing0_Sea_Salt["Sea Salt"]
CultureMech_003597 --> ing0_Sea_Salt
ing1_Yeast_extract["Yeast extract"]
CultureMech_003597 --> ing1_Yeast_extract
ing2_Sodium_resazurin["Sodium resazurin"]
CultureMech_003597 --> ing2_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Sodium_resazurin -.-> CHEBI_8806
ing3_Na2CO3["Na2CO3"]
CultureMech_003597 --> ing3_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing3_Na2CO3 -.-> CHEBI_29377
ing4_Na_DL_lactate["Na-DL-lactate"]
CultureMech_003597 --> ing4_Na_DL_lactate
CHEBI_75228["CHEBI:75228"]
ing4_Na_DL_lactate -.-> CHEBI_75228
ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003597 --> ing5_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing5_Na2S_x_9_H2O -.-> CHEBI_76209
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003597 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_NaCl["NaCl"]
CultureMech_003597 --> ing7_NaCl
CHEBI_26710["CHEBI:26710"]
ing7_NaCl -.-> CHEBI_26710
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003597 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003597 --> ing9_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing9_CuSO4_x_5_H2O -.-> CHEBI_31440
ing10_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_003597 --> ing10_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing10_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing11_H3BO3["H3BO3"]
CultureMech_003597 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003597 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_003597 --> ing13__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing13__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing14_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_003597 --> ing14_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing14_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing15_Na2SeO4["Na2SeO4"]
CultureMech_003597 --> ing15_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing15_Na2SeO4 -.-> CHEBI_77775
ing16_Biotin["Biotin"]
CultureMech_003597 --> ing16_Biotin
CHEBI_15956["CHEBI:15956"]
ing16_Biotin -.-> CHEBI_15956
ing17_Folic_acid["Folic acid"]
CultureMech_003597 --> ing17_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing17_Folic_acid -.-> CHEBI_27470
ing18_Thiamine_HCl["Thiamine HCl"]
CultureMech_003597 --> ing18_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing18_Thiamine_HCl -.-> CHEBI_49105
ing19_Riboflavin["Riboflavin"]
CultureMech_003597 --> ing19_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing19_Riboflavin -.-> CHEBI_17015
ing20_Nicotinic_acid["Nicotinic acid"]
CultureMech_003597 --> ing20_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing20_Nicotinic_acid -.-> CHEBI_15940
ing21_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_003597 --> ing21_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing21_Calcium_D_____pantothenate -.-> CHEBI_31345
ing22_Vitamin_B12["Vitamin B12"]
CultureMech_003597 --> ing22_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing22_Vitamin_B12 -.-> CHEBI_176843
ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003597 --> ing23_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
ing24__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_003597 --> ing24__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing24__DL__alpha_Lipoic_acid -.-> CHEBI_16494
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea Salt | 34.9301 G_PER_L | ||
| 2 | Yeast extract | 0.998004 G_PER_L | ||
| 3 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000499002 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 4 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.998004 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 5 |
Na-DL-lactate
C3H5O3.Na 112.06 g/mol
|
2.49501 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 6 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.299401 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
30 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 11 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.18 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 14 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2.8 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 15 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 16 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.1 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 17 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 18 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 19 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 20 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 21 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 22 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 23 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 24 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 25 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Solutions
Wolfe's mineral elixir
| Ingredient | Concentration | Notes |
|---|---|---|
| MnSO4 x H2O | 5 G_PER_L | |
| FeSO4 x 7 H2O | 1 G_PER_L | |
| CoCl2 x 6 H2O | 1.8 G_PER_L | |
| ZnSO4 x 7 H2O | 1.8 G_PER_L |
Wolin's vitamin solution (10x)
| Ingredient | Concentration | Notes |
|---|---|---|
| Pyridoxine hydrochloride | 0.1 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1040.1 |
| 2026-02-03T11:19:16.718969 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1040 |
| 2026-02-06T05:10:49.801245Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:20.896482Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003597 |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:35.079499+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "For DSM 17682" to "for_dsm_17682" for consistent matching |
| 2026-05-20T06:01:18.556946+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.0-7.2' -> {'min': 7.0, 'max': 7.2} |
| 2026-06-05T07:41:02.779872+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.839760+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:57.766027+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-15T00:17:30.254938+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 4 -> "Wolfe's mineral elixir" @ 1 ml/l; 1 -> "Wolin's vitamin solution (10x)" @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |
| 2026-09-13T00:00:00-07:00 | repair_komodo_1040_sylc_score10.py | RESOLVED_KOMODO_1040_SYLC_STRAIN_VARIANT | KOMODO Medium 1040.1 preserves SYLC medium components and pH 7.0-7.2 but applies the recipe for DSM 17682. |