Overview
- External ontology
- mediadive.medium:104a (source link unavailable) — ANAEROBACULUM MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'pyx_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:104b', 'original_category': 'bacterial'}
- {'name': 'anaerobaculum_thermoterrenum_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:104a', 'original_category': 'bacterial'}
- {'name': 'for_dsm_12857_dsm_13479_and_dsm_13480', 'source': 'KOMODO', 'source_id': 'komodo.medium:104b.5', 'original_category': 'bacterial'}
- {'name': 'for_dsm_13181', 'source': 'KOMODO', 'source_id': 'komodo.medium:104b.6', 'original_category': 'bacterial'}
- {'name': 'for_dsm_14760_dsm_14761_and_dsm_14762', 'source': 'KOMODO', 'source_id': 'komodo.medium:104b.7', 'original_category': 'bacterial'}
- {'name': 'for_dsm_19022', 'source': 'KOMODO', 'source_id': 'komodo.medium:104b.8', 'original_category': 'bacterial'}
- {'name': 'for_dsm_21120', 'source': 'KOMODO', 'source_id': 'komodo.medium:104b.9', 'original_category': 'bacterial'}
- {'name': 'for_dsm_21650', 'source': 'KOMODO', 'source_id': 'komodo.medium:104b.12', 'original_category': 'bacterial'}
- {'name': 'for_dsm_21761', 'source': 'KOMODO', 'source_id': 'komodo.medium:104b.11', 'original_category': 'bacterial'}
- {'name': 'for_dsm_5387', 'source': 'KOMODO', 'source_id': 'komodo.medium:104b.4', 'original_category': 'bacterial'}
- {'name': 'for_dsm_7320', 'source': 'KOMODO', 'source_id': 'komodo.medium:104b.3', 'original_category': 'bacterial'}
- {'name': 'for_dsm_753', 'source': 'KOMODO', 'source_id': 'komodo.medium:104b.2', 'original_category': 'bacterial'}
- {'name': 'for_dsm_753_dsm_6589_dsm_7320_dsm_15930_dsm_17592_dsm_19522_dsm_22681_dsm_23576_and_dsm_24553', 'source': 'KOMODO', 'source_id': 'komodo.medium:104b.1', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_15597', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_15597', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_15598', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_15598', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_15923', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_15923', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_16980', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_16980', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_17217', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_17217', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_17234', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_17234', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_17242', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_17242', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_17243', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_17243', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_17476', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_17476', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_17678', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_17678', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_18011', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_18011', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_18083', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_18083', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_18960', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_18960', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_19450', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_19450', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_19559', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_19559', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_19756', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_19756', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_19857', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_19857', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_2008', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_2008', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_20698', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_20698', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_20732', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_20732', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_21469', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_21469', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_21651', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_21651', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_21825', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_21825', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_21843', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_21843', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_21845', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_21845', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_21887', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_21887', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_22317', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_22317', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_22474', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_22474', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_22477', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_22477', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_22478', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_22478', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_22811', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_22811', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_22835', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_22835', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_22836', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_22836', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_22959', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_22959', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_23047', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_23047', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_23056', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_23056', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_23059', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_23059', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_23594', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_23594', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_25311', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_25311', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_3353', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_3353', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_3985', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_3985', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_6400', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_6400', 'original_category': 'bacterial'}
- {'name': 'medium_104_modified_for_dsm_9536', 'source': 'KOMODO', 'source_id': 'komodo.medium:104_9536', 'original_category': 'bacterial'}
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104a.pdf
Composition
flowchart LR
CultureMech_000474["anaerobaculum_medium"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_000474 --> ing0_Trypticase_peptone
ing1_Meat_peptone["Meat peptone"]
CultureMech_000474 --> ing1_Meat_peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_000474 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_000474 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_000474 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_000474 --> ing5_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing5_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing6_D_Glucose["D-Glucose"]
CultureMech_000474 --> ing6_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing6_D_Glucose -.-> CHEBI_17634
ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_000474 --> ing7_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000474 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000474 --> ing9_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
ing10_K2HPO4["K2HPO4"]
CultureMech_000474 --> ing10_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing10_K2HPO4 -.-> CHEBI_131527
ing11_KH2PO4["KH2PO4"]
CultureMech_000474 --> ing11_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing11_KH2PO4 -.-> CHEBI_63036
ing12_NaHCO3["NaHCO3"]
CultureMech_000474 --> ing12_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing12_NaHCO3 -.-> CHEBI_32139
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 5 G_PER_L | ||
| 2 | Meat peptone | 5 G_PER_L | ||
| 3 | Yeast extract | 10 G_PER_L | ||
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 8.0, 2.0] |
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 7 |
D-Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 8 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2.5 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 11 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 12 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 13 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Preparation
- Dissolve ingredients (except cysteine, glucose and thiosulfate), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add glucose and thiosulfate from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 7.0, if necessary.
Target organisms
No target organisms are recorded.
Provenance
Merged from 57 source records.
- KOMODO_104b_PYX-MEDIUM (CultureMech:003622)
- anaerobaculum_medium (CultureMech:000474)
- anaerobaculum_thermoterrenum_medium (CultureMech:003609)
- for_dsm_12857_dsm_13479_and_dsm_13480 (CultureMech:003617)
- for_dsm_13181 (CultureMech:003618)
- for_dsm_14760_dsm_14761_and_dsm_14762 (CultureMech:003619)
- for_dsm_19022 (CultureMech:003620)
- for_dsm_21120 (CultureMech:003621)
- for_dsm_21650 (CultureMech:003611)
- for_dsm_21761 (CultureMech:003610)
- for_dsm_5387 (CultureMech:003616)
- for_dsm_7320 (CultureMech:003615)
- for_dsm_753 (CultureMech:003614)
- for_dsm_753_dsm_6589_dsm_7320_dsm_15930_dsm_17592_dsm_19522_dsm_22681_dsm_23576_and_dsm_24553 (CultureMech:003613)
- medium_104_modified_for_dsm_15597 (CultureMech:003554)
- medium_104_modified_for_dsm_15598 (CultureMech:003555)
- medium_104_modified_for_dsm_15923 (CultureMech:003556)
- medium_104_modified_for_dsm_16980 (CultureMech:003557)
- medium_104_modified_for_dsm_17217 (CultureMech:003558)
- medium_104_modified_for_dsm_17234 (CultureMech:003559)
- medium_104_modified_for_dsm_17242 (CultureMech:003560)
- medium_104_modified_for_dsm_17243 (CultureMech:003561)
- medium_104_modified_for_dsm_17476 (CultureMech:003562)
- medium_104_modified_for_dsm_17678 (CultureMech:003563)
- medium_104_modified_for_dsm_18011 (CultureMech:003564)
- medium_104_modified_for_dsm_18083 (CultureMech:003565)
- medium_104_modified_for_dsm_18960 (CultureMech:003566)
- medium_104_modified_for_dsm_19450 (CultureMech:003567)
- medium_104_modified_for_dsm_19559 (CultureMech:003568)
- medium_104_modified_for_dsm_19756 (CultureMech:003569)
- medium_104_modified_for_dsm_19857 (CultureMech:003570)
- medium_104_modified_for_dsm_2008 (CultureMech:003571)
- medium_104_modified_for_dsm_20698 (CultureMech:003572)
- medium_104_modified_for_dsm_20732 (CultureMech:003573)
- medium_104_modified_for_dsm_21469 (CultureMech:003574)
- medium_104_modified_for_dsm_21651 (CultureMech:003575)
- medium_104_modified_for_dsm_21825 (CultureMech:003576)
- medium_104_modified_for_dsm_21843 (CultureMech:003577)
- medium_104_modified_for_dsm_21845 (CultureMech:003578)
- medium_104_modified_for_dsm_21887 (CultureMech:003579)
- medium_104_modified_for_dsm_22317 (CultureMech:003580)
- medium_104_modified_for_dsm_22474 (CultureMech:003581)
- medium_104_modified_for_dsm_22477 (CultureMech:003582)
- medium_104_modified_for_dsm_22478 (CultureMech:003583)
- medium_104_modified_for_dsm_22811 (CultureMech:003584)
- medium_104_modified_for_dsm_22835 (CultureMech:003585)
- medium_104_modified_for_dsm_22836 (CultureMech:003586)
- medium_104_modified_for_dsm_22959 (CultureMech:003587)
- medium_104_modified_for_dsm_23047 (CultureMech:003588)
- medium_104_modified_for_dsm_23056 (CultureMech:003589)
- medium_104_modified_for_dsm_23059 (CultureMech:003590)
- medium_104_modified_for_dsm_23594 (CultureMech:003591)
- medium_104_modified_for_dsm_25311 (CultureMech:003592)
- medium_104_modified_for_dsm_3353 (CultureMech:003593)
- medium_104_modified_for_dsm_3985 (CultureMech:003594)
- medium_104_modified_for_dsm_6400 (CultureMech:003595)
- medium_104_modified_for_dsm_9536 (CultureMech:003596)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.172079Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 104a |
| 2026-03-13T03:09:25.193720Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:55.627123Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000474 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.633101+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ANAEROBACULUM MEDIUM" to "anaerobaculum_medium" for consistent matching |
| 2026-06-05T07:40:48.762445+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.127240+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:38.623583+00:00 | recipe-merger | Merged 57 duplicate recipes into canonical record | Sources: KOMODO_104b_PYX-MEDIUM, anaerobaculum_medium, anaerobaculum_thermoterrenum_medium, for_dsm_12857_dsm_13479_and_dsm_13480, for_dsm_13181, for_dsm_14760_dsm_14761_and_dsm_14762, for_dsm_19022, for_dsm_21120, for_dsm_21650, for_dsm_21761, for_dsm_5387, for_dsm_7320, for_dsm_753, for_dsm_753_dsm_6589_dsm_7320_dsm_15930_dsm_17592_dsm_19522_dsm_22681_dsm_23576_and_dsm_24553, medium_104_modified_for_dsm_15597, medium_104_modified_for_dsm_15598, medium_104_modified_for_dsm_15923, medium_104_modified_for_dsm_16980, medium_104_modified_for_dsm_17217, medium_104_modified_for_dsm_17234, medium_104_modified_for_dsm_17242, medium_104_modified_for_dsm_17243, medium_104_modified_for_dsm_17476, medium_104_modified_for_dsm_17678, medium_104_modified_for_dsm_18011, medium_104_modified_for_dsm_18083, medium_104_modified_for_dsm_18960, medium_104_modified_for_dsm_19450, medium_104_modified_for_dsm_19559, medium_104_modified_for_dsm_19756, medium_104_modified_for_dsm_19857, medium_104_modified_for_dsm_2008, medium_104_modified_for_dsm_20698, medium_104_modified_for_dsm_20732, medium_104_modified_for_dsm_21469, medium_104_modified_for_dsm_21651, medium_104_modified_for_dsm_21825, medium_104_modified_for_dsm_21843, medium_104_modified_for_dsm_21845, medium_104_modified_for_dsm_21887, medium_104_modified_for_dsm_22317, medium_104_modified_for_dsm_22474, medium_104_modified_for_dsm_22477, medium_104_modified_for_dsm_22478, medium_104_modified_for_dsm_22811, medium_104_modified_for_dsm_22835, medium_104_modified_for_dsm_22836, medium_104_modified_for_dsm_22959, medium_104_modified_for_dsm_23047, medium_104_modified_for_dsm_23056, medium_104_modified_for_dsm_23059, medium_104_modified_for_dsm_23594, medium_104_modified_for_dsm_25311, medium_104_modified_for_dsm_3353, medium_104_modified_for_dsm_3985, medium_104_modified_for_dsm_6400, medium_104_modified_for_dsm_9536 |
| 2026-08-06T03:04:38.623840+00:00 | merge_recipes.py | MERGED_RECIPES | merged 57 source recipe(s) on fingerprint 7e498ca67c37850b59b33b2bc679fffb2d43f304206aef374fcb1f6ff36a6247 |