Overview
- External ontology
- komodo.medium:104b (source link unavailable) — PYX-MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: KOMODO ModelSEED | ID: 104b | DSMZ Medium: 104b (mediadive.medium:104b) | Aerobic: Yes
Composition
flowchart LR
CultureMech_003622["pyx_medium"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_003622 --> ing0_Trypticase_peptone
ing1_Meat_peptone["Meat peptone"]
CultureMech_003622 --> ing1_Meat_peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_003622 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_003622 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_003622 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003622 --> ing5_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing5_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing6_D_Glucose["D-Glucose"]
CultureMech_003622 --> ing6_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing6_D_Glucose -.-> CHEBI_17634
ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_003622 --> ing7_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003622 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003622 --> ing9_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
ing10_K2HPO4["K2HPO4"]
CultureMech_003622 --> ing10_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing10_K2HPO4 -.-> CHEBI_131527
ing11_KH2PO4["KH2PO4"]
CultureMech_003622 --> ing11_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing11_KH2PO4 -.-> CHEBI_63036
ing12_NaHCO3["NaHCO3"]
CultureMech_003622 --> ing12_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing12_NaHCO3 -.-> CHEBI_32139
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 5 G_PER_L | ||
| 2 | Meat peptone | 5 G_PER_L | ||
| 3 | Yeast extract | 10 G_PER_L | ||
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 8.0, 2.0] |
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 7 |
D-Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 8 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2.5 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 11 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 12 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 13 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 104b |
| 2026-02-03T11:18:55.839446 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 104 |
| 2026-03-13T03:08:41.794616Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:21.090304Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003622 |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:35.297922+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYX-MEDIUM" to "pyx_medium" for consistent matching |
| 2026-06-05T07:40:18.503852+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.329479+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-13T00:00:00-07:00 | repair_komodo_104b_pyx_ph_score10.py | RESOLVED_KOMODO_104B_PYX_PH_VARIANTS | Changed DSM 7320 and DSM 21650 from SOURCE_DUPLICATE to PH_VARIANT children of PYX-MEDIUM. |
| 2026-09-13T00:00:00-07:00 | repair_komodo_104b_pyx_ph_score10.py | RESOLVED_KOMODO_104B_PYX_STRAIN_VARIANTS | Changed DSM 753, DSM 5387, DSM 13181, DSM 19022, DSM 21120, and DSM 21761 from SOURCE_DUPLICATE to STRAIN_SPECIFIC_VARIANT children of PYX-MEDIUM. |