ph_medium

CultureMech:000511 bacterial COMPLEX LIQUID
Original name: PH MEDIUM

Overview

External ontology
mediadive.medium:1077 (source link unavailable) — PH MEDIUM
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1077.pdf

Composition

flowchart LR
    CultureMech_000511["ph_medium"]
    ing0_PPLO_broth["PPLO broth"]
    CultureMech_000511 --> ing0_PPLO_broth
    ing1_Horse_serum["Horse serum"]
    CultureMech_000511 --> ing1_Horse_serum
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_000511 --> ing2_Yeast_extract
    ing3_Fish_Sperm_DNA["Fish-Sperm DNA"]
    CultureMech_000511 --> ing3_Fish_Sperm_DNA
    CHEBI_16991["CHEBI:16991"]
    ing3_Fish_Sperm_DNA -.-> CHEBI_16991

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 PPLO broth 21 G_PER_L
2 Horse serum 18.7 G_PER_L
3 Yeast extract 0.234 G_PER_L
4 Fish-Sperm DNA 0.187 G_PER_L CHEBI:16991
deoxyribonucleic acid

Preparation

  1. Combine solutions 1 and 2.
  2. Adjust pH to 6.5 and autoclave.
  3. Adjust pH to 7.8.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:13.311138Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1077
2026-01-28T19:48:33.301654Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:55.957963Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000511
2026-03-17T06:30:35.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.982389+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PH MEDIUM" to "ph_medium" for consistent matching
2026-06-05T07:41:41.677744+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:52.829230+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint bcd8768f56bdc12fc335fd8ffd04e4529ec417a6624fa67bdb0685704407268a