Overview
- External ontology
- mediadive.medium:1077 (source link unavailable) — PH MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- DSMZ Medium 1077 prepares PH MEDIUM by combining 80 ml Solution 1 with 20 ml Solution 2. Solution 1 is PPLO broth in distilled water adjusted to pH 6.5 and autoclaved; Solution 2 contains inactivated horse serum, autoclaved 25% yeast extract solution, filter-sterilized fish sperm DNA solution, and water adjusted to pH 7.8.
Ingredients (0)
No ingredients recorded.
Preparation
- Prepare Solution 1, adjust it to pH 6.5, and autoclave it.
- Prepare Solution 2 and adjust it to pH 7.8.
- Combine 80 ml Solution 1 with 20 ml Solution 2.
Solutions
Solution 1
Dose: 800.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| PPLO broth | 21.0 G_PER_L | DSMZ Medium 1077 Solution 1 lists 1.68 g PPLO broth in 80.00 ml. PPLO broth is retained ungrounded because the pinned MIM label index marks it as an intentionally unmapped complex product. |
| Distilled water | 1000.0 ML_PER_L | DSMZ Medium 1077 Solution 1 lists 80.00 ml distilled water per 80 ml. |
The final 100 ml PH MEDIUM combines 80 ml Solution 1 with 20 ml Solution 2.
Solution 2
Dose: 200.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Horse serum | 935.0 ML_PER_L | DSMZ Medium 1077 Solution 2 lists 18.700 ml inactivated horse serum in 20 ml. |
| Distilled water | 9.35 ML_PER_L | DSMZ Medium 1077 Solution 2 lists 0.187 ml distilled water in 20 ml. |
The final 100 ml PH MEDIUM combines 80 ml Solution 1 with 20 ml Solution 2.
Yeast Extract Solution (25%, autoclaved)
Dose: 46.8 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Yeast extract | 250.0 G_PER_L | The stock name states 25% Yeast Extract Solution. |
DSMZ Medium 1077 Solution 2 lists 0.936 ml 25% autoclaved yeast extract solution in 20 ml.
Fish sperm DNA solution (filter-sterilized)
Dose: 9.35 ML_PER_L
DSMZ Medium 1077 Solution 2 lists 0.187 ml filter-sterilized fish sperm DNA solution in 20 ml; the source does not disclose its DNA concentration.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.311138Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1077 |
| 2026-01-28T19:48:33.301654Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:55.957963Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000511 |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.982389+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PH MEDIUM" to "ph_medium" for consistent matching |
| 2026-06-05T07:41:41.677744+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_mediadive_1077_ph_medium_score15.py | RESOLVED_MEDIADIVE_1077_PH_MEDIUM_SCORE15 | Restored the DSMZ/MediaDive Solution 1 and Solution 2 stock structure, corrected milliliter stock additions imported as g/L, grounded exact MIM-supported components, and retained PPLO broth and fish sperm DNA solution as intentionally unmapped. |