Overview
- External ontology
- mediadive.medium:1080 (source link unavailable) — MYCOPLASMA MEDIUM (CIP MEDIUM 89)
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.4}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1080.pdf
Composition
flowchart LR
CultureMech_000514["mycoplasma_medium_cip_medium_89"]
ing0_PPLO_broth["PPLO broth"]
CultureMech_000514 --> ing0_PPLO_broth
ing1_Yeast_extract["Yeast extract"]
CultureMech_000514 --> ing1_Yeast_extract
ing2_Phenol_red["Phenol red"]
CultureMech_000514 --> ing2_Phenol_red
CHEBI_31991["CHEBI:31991"]
ing2_Phenol_red -.-> CHEBI_31991
ing3_Horse_serum["Horse serum"]
CultureMech_000514 --> ing3_Horse_serum
ing4_Ampicillin["Ampicillin"]
CultureMech_000514 --> ing4_Ampicillin
CHEBI_28971["CHEBI:28971"]
ing4_Ampicillin -.-> CHEBI_28971
ing5_L_Arginine_x_HCl["L-Arginine x HCl"]
CultureMech_000514 --> ing5_L_Arginine_x_HCl
CHEBI_31235["CHEBI:31235"]
ing5_L_Arginine_x_HCl -.-> CHEBI_31235
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | PPLO broth | 17 G_PER_L | ||
| 2 | Yeast extract | 25 G_PER_L | ||
| 3 |
Phenol red
C19H14O5S 354.383 g/mol
|
0.2 G_PER_L |
CHEBI:31991
phenol red
|
|
| 4 | Horse serum | 200 G_PER_L | ||
| 5 |
Ampicillin
C16H19N3O4S 349.412 g/mol
|
4.7619 G_PER_L |
CHEBI:28971
ampicillin
|
|
| 6 |
L-Arginine x HCl
C6H14N4O2.HCl 210.665 g/mol
|
500 G_PER_L |
CHEBI:31235
Arginine hydrochloride
|
Preparation
- Add all components except sterile supplement; adjust pH to 7.2 + 0.2. Autoclave at 121°C for 15 minutes; add aseptically sterile supplement.
- Autoclave at 121°C for 15 minutes.
- 50 g L-Arginine hydrochloride plus distilled water to make up to 100 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mycoplasma_medium_cip_medium_89 (CultureMech:000514)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.316539Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1080 |
| 2026-01-28T19:48:19.247756Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:55.972058Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000514 |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.997400+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MYCOPLASMA MEDIUM (CIP MEDIUM 89)" to "mycoplasma_medium_cip_medium_89" for consistent matching |
| 2026-05-20T06:02:00.570794+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7-7.4' -> {'min': 7.0, 'max': 7.4} |
| 2026-06-05T07:41:39.040872+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:50.924135+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d3015f9dd36524303b6c4126660ed272420da6d76554e35dce3d36e3b27f8139 |