mycoplasma_medium_cip_medium_89

CultureMech:000514 bacterial COMPLEX LIQUID
Original name: MYCOPLASMA MEDIUM (CIP MEDIUM 89)

Overview

External ontology
mediadive.medium:1080 (source link unavailable) — MYCOPLASMA MEDIUM (CIP MEDIUM 89)
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 7.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1080.pdf

Composition

flowchart LR
    CultureMech_000514["mycoplasma_medium_cip_medium_89"]
    ing0_PPLO_broth["PPLO broth"]
    CultureMech_000514 --> ing0_PPLO_broth
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000514 --> ing1_Yeast_extract
    ing2_Phenol_red["Phenol red"]
    CultureMech_000514 --> ing2_Phenol_red
    CHEBI_31991["CHEBI:31991"]
    ing2_Phenol_red -.-> CHEBI_31991
    ing3_Horse_serum["Horse serum"]
    CultureMech_000514 --> ing3_Horse_serum
    ing4_Ampicillin["Ampicillin"]
    CultureMech_000514 --> ing4_Ampicillin
    CHEBI_28971["CHEBI:28971"]
    ing4_Ampicillin -.-> CHEBI_28971
    ing5_L_Arginine_x_HCl["L-Arginine x HCl"]
    CultureMech_000514 --> ing5_L_Arginine_x_HCl
    CHEBI_31235["CHEBI:31235"]
    ing5_L_Arginine_x_HCl -.-> CHEBI_31235

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 PPLO broth 17 G_PER_L
2 Yeast extract 25 G_PER_L
3 Phenol red
C19H14O5S 354.383 g/mol
0.2 G_PER_L CHEBI:31991
phenol red
4 Horse serum 200 G_PER_L
5 Ampicillin
C16H19N3O4S 349.412 g/mol
4.7619 G_PER_L CHEBI:28971
ampicillin
6 L-Arginine x HCl
C6H14N4O2.HCl 210.665 g/mol
500 G_PER_L CHEBI:31235
Arginine hydrochloride

Preparation

  1. Add all components except sterile supplement; adjust pH to 7.2 + 0.2. Autoclave at 121°C for 15 minutes; add aseptically sterile supplement.
  2. Autoclave at 121°C for 15 minutes.
  3. 50 g L-Arginine hydrochloride plus distilled water to make up to 100 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.316539Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1080
2026-01-28T19:48:19.247756Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:55.972058Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000514
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.997400+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MYCOPLASMA MEDIUM (CIP MEDIUM 89)" to "mycoplasma_medium_cip_medium_89" for consistent matching
2026-05-20T06:02:00.570794+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-7.4' -> {'min': 7.0, 'max': 7.4}
2026-06-05T07:41:39.040872+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.