Overview
- External ontology
- mediadive.medium:1115 (source link unavailable) — CONGREGIBACTER (SYPHC) MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.3, 'max': 7.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1115.pdf
Composition
flowchart LR
CultureMech_000551["congregibacter_syphc_medium"]
ing0_Sea_Salt["Sea Salt"]
CultureMech_000551 --> ing0_Sea_Salt
ing1_NH4Cl["NH4Cl"]
CultureMech_000551 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_Yeast_extract["Yeast extract"]
CultureMech_000551 --> ing2_Yeast_extract
ing3_Na_pyruvate["Na-pyruvate"]
CultureMech_000551 --> ing3_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing3_Na_pyruvate -.-> CHEBI_50144
ing4_HEPES["HEPES"]
CultureMech_000551 --> ing4_HEPES
CHEBI_46756["CHEBI:46756"]
ing4_HEPES -.-> CHEBI_46756
ing5_L_Histidine["L-Histidine"]
CultureMech_000551 --> ing5_L_Histidine
CHEBI_15971["CHEBI:15971"]
ing5_L_Histidine -.-> CHEBI_15971
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_000551 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_KH2PO4["KH2PO4"]
CultureMech_000551 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000551 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_000551 --> ing9_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing9_MnSO4_x_H2O -.-> CHEBI_86364
ing10_NaCl["NaCl"]
CultureMech_000551 --> ing10_NaCl
CHEBI_26710["CHEBI:26710"]
ing10_NaCl -.-> CHEBI_26710
ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000551 --> ing11_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000551 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000551 --> ing13_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000551 --> ing14_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000551 --> ing15_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
ing16_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_000551 --> ing16_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing16_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing17_H3BO3["H3BO3"]
CultureMech_000551 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000551 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_000551 --> ing19__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing19__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing20_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_000551 --> ing20_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing20_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing21_Na2SeO4["Na2SeO4"]
CultureMech_000551 --> ing21_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing21_Na2SeO4 -.-> CHEBI_77775
ing22_Vitamin_B12["Vitamin B12"]
CultureMech_000551 --> ing22_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing22_Vitamin_B12 -.-> CHEBI_176843
ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_000551 --> ing23_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
ing24_D_____biotin["D-(+)-biotin"]
CultureMech_000551 --> ing24_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing24_D_____biotin -.-> CHEBI_15956
ing25_Nicotinic_acid["Nicotinic acid"]
CultureMech_000551 --> ing25_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing25_Nicotinic_acid -.-> CHEBI_15940
ing26_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_000551 --> ing26_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing26_Calcium_pantothenate -.-> CHEBI_31345
ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000551 --> ing27_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing28_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_000551 --> ing28_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing28_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea Salt | 34.965 G_PER_L | ||
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.0999001 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 | Yeast extract | 0.999001 G_PER_L | ||
| 4 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
1.0989 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 5 | HEPES | 2.4975 G_PER_L |
CHEBI:46756
HEPES
|
|
| 6 |
L-Histidine
C6H9N3O2 155.157 g/mol
|
0.03996 G_PER_L |
CHEBI:15971
L-histidine
|
|
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.03996 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.04995 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
30 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 10 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 12 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1.8 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 14 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 15 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.8 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 16 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 17 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.18 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2.8 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 21 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 22 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.1 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 23 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 24 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 25 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 26 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.2 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 27 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 28 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.3 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 29 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.2 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Preparation
- Dissolve ingredients (except vitamins and hydrogenphosphate) and adjust pH to 7.7. After autoclaving add vitamins and hydrogenphosphate from sterile stock solutions sterilized by filtration. Adjust pH of complete medium to 7.3 - 7.5, if necessary.
- Note: Growth of most strains is stimulated by using microaerophilic conditions with only 10% (v/v) oxygen in the head space gas atmosphere. For microaerophilic growth medium is prepared without HEPES, sparged with 80% N2 and 20% CO2 gas mixture to make it anoxic and then dispensed under air atmosphere into serum vials to 50% volume. After autoclaving the pH is adjusted to 7.5 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas mixture.
- First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- congregibacter_syphc_medium (CultureMech:000551)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.426565Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1115 |
| 2026-03-13T06:18:56.321837Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000551 |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.389417+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CONGREGIBACTER (SYPHC) MEDIUM" to "congregibacter_syphc_medium" for consistent matching |
| 2026-05-20T06:01:12.476489+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.3-7.5' -> {'min': 7.3, 'max': 7.5} |
| 2026-06-05T07:40:54.789215+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.657726+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:54.970076+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:29.102056+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 88f1e94c26b6fbfbf65a85d445e804cadcac1794084d4651b07f055cad7f59ba |