congregibacter_syphc_medium

Overview

External ontology

mediadive.medium:1115 (source link unavailable) — CONGREGIBACTER (SYPHC) MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 7.3, 'max': 7.5}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1115.pdf

Composition

flowchart LR
    CultureMech_000551["congregibacter_syphc_medium"]
    ing0_Sea_Salt["Sea Salt"]
    CultureMech_000551 --> ing0_Sea_Salt
    ing1_NH4Cl["NH4Cl"]
    CultureMech_000551 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_000551 --> ing2_Yeast_extract
    ing3_Na_pyruvate["Na-pyruvate"]
    CultureMech_000551 --> ing3_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing3_Na_pyruvate -.-> CHEBI_50144
    ing4_HEPES["HEPES"]
    CultureMech_000551 --> ing4_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing4_HEPES -.-> CHEBI_46756
    ing5_L_Histidine["L-Histidine"]
    CultureMech_000551 --> ing5_L_Histidine
    CHEBI_15971["CHEBI:15971"]
    ing5_L_Histidine -.-> CHEBI_15971
    ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000551 --> ing6_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing7_KH2PO4["KH2PO4"]
    CultureMech_000551 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000551 --> ing8_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing9_NaCl["NaCl"]
    CultureMech_000551 --> ing9_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing9_NaCl -.-> CHEBI_26710
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000551 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000551 --> ing11_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing12_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_000551 --> ing12_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing12_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing13_H3BO3["H3BO3"]
    CultureMech_000551 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000551 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_000551 --> ing15__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing15__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing16_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000551 --> ing16_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing16_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing17_Na2SeO4["Na2SeO4"]
    CultureMech_000551 --> ing17_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing17_Na2SeO4 -.-> CHEBI_77775
    ing18_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000551 --> ing18_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing18_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing19_D_____biotin["D-(+)-biotin"]
    CultureMech_000551 --> ing19_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_D_____biotin -.-> CHEBI_15956
    ing20_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000551 --> ing20_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing20_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (21)

Preparation

1. Dissolve ingredients (except vitamins and hydrogenphosphate) and adjust pH to 7.7. After autoclaving add vitamins and hydrogenphosphate from sterile stock solutions sterilized by filtration. Adjust pH of complete medium to 7.3 - 7.5, if necessary.
2. Note: Growth of most strains is stimulated by using microaerophilic conditions with only 10% (v/v) oxygen in the head space gas atmosphere. For microaerophilic growth medium is prepared without HEPES, sparged with 80% N2 and 20% CO2 gas mixture to make it anoxic and then dispensed under air atmosphere into serum vials to 50% volume. After autoclaving the pH is adjusted to 7.5 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas mixture.
3. First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.

Solutions

Target organisms

No target organisms are recorded.

Provenance