Overview
- External ontology
- mediadive.medium:1135 (source link unavailable) — HD-MEDIUM, 1:10 diluted, modified
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1135.pdf
Composition
flowchart LR
CultureMech_000571["hd_medium_1_10_diluted_modified"]
ing0_Casein_peptone["Casein peptone"]
CultureMech_000571 --> ing0_Casein_peptone
ing1_Glucose["Glucose"]
CultureMech_000571 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_Yeast_extract["Yeast extract"]
CultureMech_000571 --> ing2_Yeast_extract
ing3_MES["MES"]
CultureMech_000571 --> ing3_MES
CHEBI_39010["CHEBI:39010"]
ing3_MES -.-> CHEBI_39010
ing4_Double_distilled_water["Double distilled water"]
CultureMech_000571 --> ing4_Double_distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Double_distilled_water -.-> CHEBI_15377
ing5_Agar["Agar"]
CultureMech_000571 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein peptone | 0.5 G_PER_L | ||
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
0.1 G_PER_L |
CHEBI:17234
glucose
|
|
| 3 | Yeast extract | 0.25 G_PER_L | ||
| 4 | MES | 1.95 G_PER_L |
CHEBI:39010
MES
|
|
| 5 |
Double distilled water
H2O 18.015 g/mol
|
2000.0 G_PER_L |
CHEBI:15377
water
|
[Merged 3 duplicates: 1000.0, 500.0, 500.0] |
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust to pH 5.5 (with NaOh/HCl)
- Sterilize separately. For solid medium combine solution A with solution B at 50°C.
- Liquid and solid medium possible. Incubation at 20°C, 4 days.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- hd_medium_1_10_diluted_modified (CultureMech:000571)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.479899Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1135 |
| 2026-03-13T03:08:38.431634Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:18:56.464914Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000571 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.554485+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HD-MEDIUM, 1:10 diluted, modified" to "hd_medium_1_10_diluted_modified" for consistent matching |
| 2026-06-05T07:41:08.333841+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:37.422937+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 33364d2dc3ebad2b73eeb38fb13fcd722150d07bdb52494fb6140862853ecda4 |