hd_medium_1_10_diluted_modified

CultureMech:000571 bacterial COMPLEX SOLID_AGAR
Original name: HD-MEDIUM, 1:10 diluted, modified

Overview

External ontology
mediadive.medium:1135 (source link unavailable) — HD-MEDIUM, 1:10 diluted, modified
Applications
  • Microbial cultivation
pH
5.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1135.pdf

Composition

flowchart LR
    CultureMech_000571["hd_medium_1_10_diluted_modified"]
    ing0_Casein_peptone["Casein peptone"]
    CultureMech_000571 --> ing0_Casein_peptone
    ing1_Glucose["Glucose"]
    CultureMech_000571 --> ing1_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing1_Glucose -.-> CHEBI_17234
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_000571 --> ing2_Yeast_extract
    ing3_MES["MES"]
    CultureMech_000571 --> ing3_MES
    CHEBI_39010["CHEBI:39010"]
    ing3_MES -.-> CHEBI_39010
    ing4_Double_distilled_water["Double distilled water"]
    CultureMech_000571 --> ing4_Double_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Double_distilled_water -.-> CHEBI_15377
    ing5_Agar["Agar"]
    CultureMech_000571 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Casein peptone 0.5 G_PER_L
2 Glucose
C6H12O6 180.156 g/mol
0.1 G_PER_L CHEBI:17234
glucose
3 Yeast extract 0.25 G_PER_L
4 MES 1.95 G_PER_L CHEBI:39010
MES
5 Double distilled water
H2O 18.015 g/mol
2000.0 G_PER_L CHEBI:15377
water
 [Merged 3 duplicates: 1000.0, 500.0, 500.0]
6 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust to pH 5.5 (with NaOh/HCl)
  2. Sterilize separately. For solid medium combine solution A with solution B at 50°C.
  3. Liquid and solid medium possible. Incubation at 20°C, 4 days.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.479899Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1135
2026-03-13T03:08:38.431634Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:18:56.464914Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000571
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.554485+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HD-MEDIUM, 1:10 diluted, modified" to "hd_medium_1_10_diluted_modified" for consistent matching
2026-06-05T07:41:08.333841+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.