methylonatrum_medium

CultureMech:000612 bacterial DEFINED LIQUID
Original name: METHYLONATRUM MEDIUM

Overview

External ontology
mediadive.medium:1170 (source link unavailable) — METHYLONATRUM MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'methylonatrum_medium_replace_methanol_with_sodium_acetate', 'source': 'KOMODO', 'source_id': 'komodo.medium:1170_replace_Methanol_with_Sodium acetate', 'original_category': 'bacterial'}
pH
10.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1170.pdf

Composition

flowchart LR
    CultureMech_000612["methylonatrum_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_000612 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KNO3["KNO3"]
    CultureMech_000612 --> ing1_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing1_KNO3 -.-> CHEBI_63043
    ing2_Na2CO3["Na2CO3"]
    CultureMech_000612 --> ing2_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing2_Na2CO3 -.-> CHEBI_29377
    ing3_Methanol["Methanol"]
    CultureMech_000612 --> ing3_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing3_Methanol -.-> CHEBI_17790
    ing4_NaCl["NaCl"]
    CultureMech_000612 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_NH4Cl["NH4Cl"]
    CultureMech_000612 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000612 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_NaS2O3_x_5_H2O["NaS2O3 x 5 H2O"]
    CultureMech_000612 --> ing7_NaS2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing7_NaS2O3_x_5_H2O -.-> CHEBI_32150
    ing8_Vitamin_B12["Vitamin B12"]
    CultureMech_000612 --> ing8_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing8_Vitamin_B12 -.-> CHEBI_176843
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000612 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000612 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_000612 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000612 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000612 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000612 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000612 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 KNO3
K.NO3 101.102 g/mol
1 G_PER_L CHEBI:63043
potassium nitrate
3 Na2CO3
CO3.2Na 105.988 g/mol
11 G_PER_L CHEBI:29377
sodium carbonate
4 Methanol
CH4O 32.042 g/mol
1.584 G_PER_L CHEBI:17790
methanol
5 NaCl
Cl.Na 58.443 g/mol
18 G_PER_L CHEBI:26710
sodium chloride
6 NH4Cl
Cl.H4N 53.492 g/mol
0.16 G_PER_L CHEBI:31206
ammonium chloride
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.24 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
8 NaS2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
0.024 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
9 Vitamin B12 0.1 G_PER_L CHEBI:176843
vitamin B12
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. pH 10.0
  2. Prepare the medium without methanol, vitamin B12, MgSO4 x 7 H2O and NaS2O3 x 5 H2O in closed vessels with a medium to headspace ratio of 1:5 to 1:10. Add the methanol, MgSO4 x 7 H2O and NaS2O3 x 5 H2O from autoclaved stock solutions after sterilisation of the medium. Vitamin B12 is added from a filter sterilised stock solution to the sterile medium. Incubate the strains with shaking at 150 rpm. Growth is faster in the presence of 20 mM sodium acetate instead of methanol.

Target organisms

No target organisms are recorded.

Provenance

Merged from 3 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:13.580603Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1170
2026-01-28T19:48:14.516881Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:56.809072Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000612
2026-03-17T06:29:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.924168+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLONATRUM MEDIUM" to "methylonatrum_medium" for consistent matching
2026-06-05T07:40:16.020986+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.055373+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:42.601376+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:34.376178+00:00 recipe-merger Merged 3 duplicate recipes into canonical record Sources: DSMZ_1170_METHYLONATRUM_MEDIUM, KOMODO_1170_METHYLONATRUM_MEDIUM, methylonatrum_medium_replace_methanol_with_sodium_acetate
2026-08-06T03:04:34.376224+00:00 merge_recipes.py MERGED_RECIPES merged 3 source recipe(s) on fingerprint a0ded40a4d15edb5ae8f20b0566359f90d5f26ddff270f3cfc0929699484588a