spirochaeta_cellobiosiphila_medium

CultureMech:000630 bacterial COMPLEX LIQUID
Original name: SPIROCHAETA CELLOBIOSIPHILA MEDIUM

Overview

External ontology
mediadive.medium:1188 (source link unavailable) — SPIROCHAETA CELLOBIOSIPHILA MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'sypc_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:1188', 'original_category': 'bacterial'}
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1188.pdf

Composition

flowchart LR
    CultureMech_000630["spirochaeta_cellobiosiphila_medium"]
    ing0_Sea_Salt["Sea Salt"]
    CultureMech_000630 --> ing0_Sea_Salt
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000630 --> ing1_Yeast_extract
    ing2_Trypticase_peptone["Trypticase peptone"]
    CultureMech_000630 --> ing2_Trypticase_peptone
    ing3_Cellobiose["Cellobiose"]
    CultureMech_000630 --> ing3_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing3_Cellobiose -.-> CHEBI_17057
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000630 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000630 --> ing5_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing5_MnSO4_x_H2O -.-> CHEBI_86364
    ing6_NaCl["NaCl"]
    CultureMech_000630 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000630 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000630 --> ing8_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000630 --> ing9_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000630 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000630 --> ing11_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing12_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_000630 --> ing12_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing12_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing13_H3BO3["H3BO3"]
    CultureMech_000630 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000630 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_000630 --> ing15__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing15__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing16_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000630 --> ing16_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing16_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing17_Na2SeO4["Na2SeO4"]
    CultureMech_000630 --> ing17_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing17_Na2SeO4 -.-> CHEBI_77775

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 Sea Salt 34.965 G_PER_L
2 Yeast extract 0.999001 G_PER_L
3 Trypticase peptone 0.499501 G_PER_L
4 Cellobiose
C12H22O11 342.297 g/mol
1.998 G_PER_L CHEBI:17057
cellobiose
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
30 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
7 NaCl
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
1.8 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
10 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1 G_PER_L CHEBI:86158
calcium chloride dihydrate
11 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.8 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
12 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
13 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.18 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
14 H3BO3
H3BO3 61.833 g/mol
0.1 G_PER_L CHEBI:33118
boric acid
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
16 (NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
2.8 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
17 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
18 Na2SeO4
2Na.O4Se 188.936 g/mol
0.1 G_PER_L CHEBI:77775
sodium selenate

Preparation

  1. Dissolve compounds except cellobiose, adjust pH to 7.5 and dispense medium under air atmosphere into Hungate-type tubes or serum vials to 30% volume and autoclave. Add cellobiose after autoclaving from a stock solution sterilized by filtration.
  2. First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:13.625269Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1188
2026-03-13T06:18:56.933023Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000630
2026-03-17T06:29:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:08.075574+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SPIROCHAETA CELLOBIOSIPHILA MEDIUM" to "spirochaeta_cellobiosiphila_medium" for consistent matching
2026-06-05T07:41:46.278756+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.856618+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:11.618424+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:56.065927+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: spirochaeta_cellobiosiphila_medium, sypc_medium
2026-08-06T03:05:56.065974+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 1917aa42a85b4011e04abd57aef045cf4ac45c2cf4ebb5ffbda5da8b236b8955