Overview
- External ontology
- mediadive.medium:1188b (source link unavailable) — MARISPIROCHAETA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1188b.pdf
Composition
flowchart LR
CultureMech_000631["marispirochaeta_medium"]
ing0_Sea_Salt["Sea Salt"]
CultureMech_000631 --> ing0_Sea_Salt
ing1_Yeast_extract["Yeast extract"]
CultureMech_000631 --> ing1_Yeast_extract
ing2_Trypticase_peptone["Trypticase peptone"]
CultureMech_000631 --> ing2_Trypticase_peptone
ing3_Sodium_resazurin["Sodium resazurin"]
CultureMech_000631 --> ing3_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Sodium_resazurin -.-> CHEBI_8806
ing4_Na2CO3["Na2CO3"]
CultureMech_000631 --> ing4_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing4_Na2CO3 -.-> CHEBI_29377
ing5_Cellobiose["Cellobiose"]
CultureMech_000631 --> ing5_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing5_Cellobiose -.-> CHEBI_17057
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000631 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_000631 --> ing7_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing7_MnSO4_x_H2O -.-> CHEBI_86364
ing8_NaCl["NaCl"]
CultureMech_000631 --> ing8_NaCl
CHEBI_26710["CHEBI:26710"]
ing8_NaCl -.-> CHEBI_26710
ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000631 --> ing9_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000631 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000631 --> ing11_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000631 --> ing12_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000631 --> ing13_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
ing14_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_000631 --> ing14_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing14_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing15_H3BO3["H3BO3"]
CultureMech_000631 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000631 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_000631 --> ing17__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing17__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing18_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_000631 --> ing18_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing18_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing19_Na2SeO4["Na2SeO4"]
CultureMech_000631 --> ing19_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing19_Na2SeO4 -.-> CHEBI_77775
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea Salt | 34.965 G_PER_L | ||
| 2 | Yeast extract | 0.999001 G_PER_L | ||
| 3 | Trypticase peptone | 0.499501 G_PER_L | ||
| 4 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0004995 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 5 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.999001 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 6 |
Cellobiose
C12H22O11 342.297 g/mol
|
1.998 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
30 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 9 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 10 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1.8 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 13 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.8 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 14 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 15 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.18 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2.8 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 19 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 20 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.1 G_PER_L |
CHEBI:77775
sodium selenate
|
Preparation
- Dissolve compounds except carbonate and cellobiose, then sparge medium for 30 - 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add carbonate from an anoxic sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture and cellobiose from an anoxic stock solution prepared under 100% N2 and sterilized by filtration. Check pH of complete medium and adjust to 7.0, if necessary.
- First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- marispirochaeta_medium (CultureMech:000631)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.628120Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1188b |
| 2026-03-13T06:18:56.942040Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000631 |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.085892+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MARISPIROCHAETA MEDIUM" to "marispirochaeta_medium" for consistent matching |
| 2026-06-05T07:41:13.219593+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.083786+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:00.995016+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:42.193961+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4dcee053f0cd7ad562892e1e7b9b8b24de39ff489f735f27d9e886027fc59935 |