marispirochaeta_medium

CultureMech:000631 bacterial COMPLEX LIQUID
Original name: MARISPIROCHAETA MEDIUM

Overview

External ontology
mediadive.medium:1188b (source link unavailable) — MARISPIROCHAETA MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1188b.pdf

Composition

flowchart LR
    CultureMech_000631["marispirochaeta_medium"]
    ing0_Sea_Salt["Sea Salt"]
    CultureMech_000631 --> ing0_Sea_Salt
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000631 --> ing1_Yeast_extract
    ing2_Trypticase_peptone["Trypticase peptone"]
    CultureMech_000631 --> ing2_Trypticase_peptone
    ing3_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000631 --> ing3_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Sodium_resazurin -.-> CHEBI_8806
    ing4_Na2CO3["Na2CO3"]
    CultureMech_000631 --> ing4_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing4_Na2CO3 -.-> CHEBI_29377
    ing5_Cellobiose["Cellobiose"]
    CultureMech_000631 --> ing5_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing5_Cellobiose -.-> CHEBI_17057
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000631 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_NaCl["NaCl"]
    CultureMech_000631 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000631 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000631 --> ing9_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing9_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing10_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_000631 --> ing10_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing10_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing11_H3BO3["H3BO3"]
    CultureMech_000631 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000631 --> ing12_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing13__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_000631 --> ing13__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing13__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing14_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000631 --> ing14_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing14_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing15_Na2SeO4["Na2SeO4"]
    CultureMech_000631 --> ing15_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing15_Na2SeO4 -.-> CHEBI_77775

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Sea Salt 34.965 G_PER_L
2 Yeast extract 0.999001 G_PER_L
3 Trypticase peptone 0.499501 G_PER_L
4 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0004995 G_PER_L CHEBI:8806
Resazurin
5 Na2CO3
CO3.2Na 105.988 g/mol
0.999001 G_PER_L CHEBI:29377
sodium carbonate
6 Cellobiose
C12H22O11 342.297 g/mol
1.998 G_PER_L CHEBI:17057
cellobiose
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
30 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
8 NaCl
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
9 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1 G_PER_L CHEBI:86158
calcium chloride dihydrate
10 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
11 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.18 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.1 G_PER_L CHEBI:33118
boric acid
13 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
14 (NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
2.8 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
15 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
16 Na2SeO4
2Na.O4Se 188.936 g/mol
0.1 G_PER_L CHEBI:77775
sodium selenate

Preparation

  1. Dissolve compounds except carbonate and cellobiose, then sparge medium for 30 - 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add carbonate from an anoxic sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture and cellobiose from an anoxic stock solution prepared under 100% N2 and sterilized by filtration. Check pH of complete medium and adjust to 7.0, if necessary.
  2. First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.

Solutions

Wolfe's mineral elixir

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
MnSO4 x H2O 5 G_PER_L
FeSO4 x 7 H2O 1 G_PER_L
CoCl2 x 6 H2O 1.8 G_PER_L
ZnSO4 x 7 H2O 1.8 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.628120Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1188b
2026-03-13T06:18:56.942040Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000631
2026-03-17T06:29:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:08.085892+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MARISPIROCHAETA MEDIUM" to "marispirochaeta_medium" for consistent matching
2026-06-05T07:41:13.219593+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.083786+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:00.995016+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.502383+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 4 stock-strength component(s) out of ingredients into 1 solution(s): 4 -> "Wolfe's mineral elixir" @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).