methanobacterium_medium

Overview

External ontology

mediadive.medium:119 (source link unavailable) — METHANOBACTERIUM MEDIUM

Applications

• Microbial cultivation

Synonyms

• {'name': 'gudongella_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:119c', 'original_category': 'bacterial'}

pH range

{'min': 6.8, 'max': 7.0}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_000643["methanobacterium_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000643 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000643 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_000643 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_000643 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000643 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_000643 --> ing5_Yeast_extract
    ing6_Na_acetate["Na-acetate"]
    CultureMech_000643 --> ing6_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Na_acetate -.-> CHEBI_32954
    ing7_Na_formate["Na-formate"]
    CultureMech_000643 --> ing7_Na_formate
    CHEBI_62965["CHEBI:62965"]
    ing7_Na_formate -.-> CHEBI_62965
    ing8_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000643 --> ing8_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Sodium_resazurin -.-> CHEBI_8806
    ing9_NaHCO3["NaHCO3"]
    CultureMech_000643 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000643 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000643 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_Sludge["Sludge"]
    CultureMech_000643 --> ing12_Sludge
    ing13_Isobutyric_acid["Isobutyric acid"]
    CultureMech_000643 --> ing13_Isobutyric_acid
    CHEBI_16135["CHEBI:16135"]
    ing13_Isobutyric_acid -.-> CHEBI_16135
    ing14_Tryptone["Tryptone"]
    CultureMech_000643 --> ing14_Tryptone
    ing15_Yeast_extract["Yeast extract"]
    CultureMech_000643 --> ing15_Yeast_extract
    ing16_Sodium_chloride["Sodium chloride"]
    CultureMech_000643 --> ing16_Sodium_chloride
    ing16_Sodium_chloride -.-> CHEBI_26710
    ing17_Valeric_acid["Valeric acid"]
    CultureMech_000643 --> ing17_Valeric_acid
    CHEBI_17418["CHEBI:17418"]
    ing17_Valeric_acid -.-> CHEBI_17418
    ing18_Isovaleric_acid["Isovaleric acid"]
    CultureMech_000643 --> ing18_Isovaleric_acid
    CHEBI_28484["CHEBI:28484"]
    ing18_Isovaleric_acid -.-> CHEBI_28484
    ing19_HCl["HCl"]
    CultureMech_000643 --> ing19_HCl
    CHEBI_17883["CHEBI:17883"]
    ing19_HCl -.-> CHEBI_17883
    ing20_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000643 --> ing20_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing20_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing21_ZnCl2["ZnCl2"]
    CultureMech_000643 --> ing21_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing21_ZnCl2 -.-> CHEBI_49976
    ing22_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000643 --> ing22_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing22_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing23_H3BO3["H3BO3"]
    CultureMech_000643 --> ing23_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing23_H3BO3 -.-> CHEBI_33118
    ing24_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000643 --> ing24_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing24_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing25_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000643 --> ing25_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing25_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing26_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000643 --> ing26_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing26_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing27_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000643 --> ing27_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing27_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing28_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000643 --> ing28_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing28_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing29_H2SO4["H2SO4"]
    CultureMech_000643 --> ing29_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing29_H2SO4 -.-> CHEBI_26836

Ingredients (30)

Preparation

1. Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate, adjust pH to 6.5 and dispense medium under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary.
2. Note: After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure.
3. Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37°C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at 8-12°C in the dark.
4. Adjust pH to 7.5 with concentrated NaOH.
5. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
6. The ferrous sulfate solution is not stable and should be freshly prepared.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

• methanobacterium_medium (CultureMech:000643)
• gudongella_medium (CultureMech:000646)