methanobacterium_medium (CultureMech:000643)

Overview

External ontology

mediadive.medium:119 (source link unavailable) — METHANOBACTERIUM MEDIUM

Applications

Microbial cultivation

pH range

{'min': 6.8, 'max': 7.0}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. Archive all prior ingredient nodes, including false LB expansion, combined yeast amounts and copied or chemically inexact annotations, in append-only history. New nodes follow this exact source's stage and label; other unchanged chemical annotations are preserved, not recertified. Source-scoped recipe inputs supersede unsupported flattened concentrations and false LB identities. All prior history and permanent IDs remain unchanged; unquantified quantities remain unknown. No taxa-media association is added.

Composition

flowchart LR
    CultureMech_000643["methanobacterium_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000643 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000643 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_000643 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_000643 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000643 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_000643 --> ing5_Yeast_extract
    ing6_Na_acetate["Na-acetate"]
    CultureMech_000643 --> ing6_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Na_acetate -.-> CHEBI_32954
    ing7_Na_formate["Na-formate"]
    CultureMech_000643 --> ing7_Na_formate
    CHEBI_62965["CHEBI:62965"]
    ing7_Na_formate -.-> CHEBI_62965
    ing8_NaHCO3["NaHCO3"]
    CultureMech_000643 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000643 --> ing9_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000643 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209
    ing11_Distilled_water["Distilled water"]
    CultureMech_000643 --> ing11_Distilled_water

Ingredients (12)

#	Ingredient	Concentration	Ontology	Notes
1	KH2PO4 H2O4P.K 136.084 g/mol	0.498504 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Direct basal input 0.5 g / explicit original final volume 1.003 L = 0.498504 g/L (six significant digits). Excludes stock preparation masses and applies only to base DSMZ 119.
2	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.398804 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Direct basal input 0.4 g / explicit original final volume 1.003 L = 0.398804 g/L (six significant digits). Excludes stock preparation masses and applies only to base DSMZ 119.
3	NaCl Cl.Na 58.443 g/mol	0.398804 G_PER_L	CHEBI:26710 sodium chloride	Direct basal input 0.4 g / explicit original final volume 1.003 L = 0.398804 g/L (six significant digits). Excludes stock preparation masses and applies only to base DSMZ 119.
4	NH4Cl Cl.H4N 53.492 g/mol	0.398804 G_PER_L	CHEBI:31206 ammonium chloride	Direct basal input 0.4 g / explicit original final volume 1.003 L = 0.398804 g/L (six significant digits). Excludes stock preparation masses and applies only to base DSMZ 119.
5	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.0498504 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Direct basal input 0.05 g / explicit original final volume 1.003 L = 0.0498504 g/L (six significant digits). Excludes stock preparation masses and applies only to base DSMZ 119.
6	Yeast extract	0.997009 G_PER_L		Superseded flattening history, not a current amount: [Merged 2 duplicates: 0.997009, 4.0] Direct basal input 1 g / explicit original final volume 1.003 L = 0.997009 g/L (six significant digits). Excludes stock preparation masses and applies only to base DSMZ 119. Literal source material qualifier: OXOID. The old combined yeast amount mixed basal and sludge preparation stages. Keep their independent inputs separate; incubation and centrifugation do not prove quantitative yeast transfer to the final medium.
7	Na-acetate C2H3O2.Na 82.034 g/mol	0.997009 G_PER_L	CHEBI:32954 sodium acetate	Direct basal input 1 g / explicit original final volume 1.003 L = 0.997009 g/L (six significant digits). Excludes stock preparation masses and applies only to base DSMZ 119.
8	Na-formate CHO2.Na 68.007 g/mol	1.99402 G_PER_L	CHEBI:62965 sodium formate	Direct basal input 2 g / explicit original final volume 1.003 L = 1.99402 g/L (six significant digits). Excludes stock preparation masses and applies only to base DSMZ 119.
9	NaHCO3 CHO3.Na 84.006 g/mol	3.98804 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Direct basal input 4 g / explicit original final volume 1.003 L = 3.98804 g/L (six significant digits). Excludes stock preparation masses and applies only to base DSMZ 119.
10	L-Cysteine HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.498504 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	Direct basal input 0.5 g / explicit original final volume 1.003 L = 0.498504 g/L (six significant digits). Excludes stock preparation masses and applies only to base DSMZ 119.
11	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.498504 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Direct basal input 0.5 g / explicit original final volume 1.003 L = 0.498504 g/L (six significant digits). Excludes stock preparation masses and applies only to base DSMZ 119.
12	Distilled water	0.93 L		Preparation input: 930 ml = 0.93 L. This is input volume, not a mass, stock dose or measured final-volume denominator.

Preparation

Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate, adjust pH to 6.5 and dispense medium under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary.
Note: After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure.

Solutions

Trace element solution SL-10

Dose: 0.001 L

Stock composition
Ingredient	Concentration	Notes
HCl	0.01 L	Preparation input: 10 ml = 0.01 L. This is input volume, not a mass, stock dose or measured final-volume denominator. Literal source material qualifier: 25%. The 25% basis is unspecified; do not infer a mass percentage or density.
FeCl2 x 4 H2O	1.5 G_PER_L	Stock input 1.5 g = 1.5 g, divided by the explicitly made-up final stock volume of 1 L. Not a receiving-medium concentration.
ZnCl2	0.07 G_PER_L	Stock input 70 mg = 0.07 g, divided by the explicitly made-up final stock volume of 1 L. Not a receiving-medium concentration.
MnCl2 x 4 H2O	0.1 G_PER_L	Stock input 100 mg = 0.1 g, divided by the explicitly made-up final stock volume of 1 L. Not a receiving-medium concentration.
H3BO3	0.006 G_PER_L	Stock input 6 mg = 0.006 g, divided by the explicitly made-up final stock volume of 1 L. Not a receiving-medium concentration.
CoCl2 x 6 H2O	0.19 G_PER_L	Stock input 190 mg = 0.19 g, divided by the explicitly made-up final stock volume of 1 L. Not a receiving-medium concentration.
CuCl2 x 2 H2O	0.002 G_PER_L	Stock input 2 mg = 0.002 g, divided by the explicitly made-up final stock volume of 1 L. Not a receiving-medium concentration.
NiCl2 x 6 H2O	0.024 G_PER_L	Stock input 24 mg = 0.024 g, divided by the explicitly made-up final stock volume of 1 L. Not a receiving-medium concentration. Copied annotations do not establish the source's exact chemical form; withdraw them, preserve the literal source name, and archive the prior node. No replacement ontology identifier is inferred.
Na2MoO4 x 2 H2O	0.036 G_PER_L	Stock input 36 mg = 0.036 g, divided by the explicitly made-up final stock volume of 1 L. Not a receiving-medium concentration.
Distilled water	0.99 L	Preparation input: 990 ml = 0.99 L. This is input volume, not a mass, stock dose or measured final-volume denominator.

Receiving-medium addition: 1 ml = 0.001 L of this prepared stock. This is an addition volume, not stock strength or final-medium concentration.

FeSO4 x 7 H2O solution (0.1% w/v)

Dose: 0.002 L

Stock composition
Ingredient	Concentration	Notes
FeSO4 x 7 H2O	0.1 PERCENT_W_V	The named stock explicitly has 0.1% w/v ferrous sulfate heptahydrate, prepared from 1 g and 1000 ml of 0.1 N sulfuric acid. The receiving dose remains separate.
H2SO4	1 L	Preparation input: 1000 ml = 1 L. This is input volume, not a mass, stock dose or measured final-volume denominator. Literal source material qualifier: 0.1 N. Retain 0.1 N as source text; no unsupported normality enum, molarity or g/L is introduced.

Receiving-medium addition: 2 ml = 0.002 L of this prepared stock. This is an addition volume, not stock strength or final-medium concentration.

Sludge fluid

Dose: 0.05 L

Stock composition
Ingredient	Concentration	Notes
Yeast extract		Superseded flattening history, not a current amount: [Merged 2 duplicates: 0.997009, 4.0] Source batch input: 4 g. Keep the mass in notes; neither input water nor API volume/g_l encoding establishes a measured final volume. No g/L concentration is asserted. The old combined yeast amount mixed basal and sludge preparation stages. Keep their independent inputs separate; incubation and centrifugation do not prove quantitative yeast transfer to the final medium.
Sludge	1 L	Preparation input: 1000 ml = 1 L. This is input volume, not a mass, stock dose or measured final-volume denominator.

Receiving-medium addition: 50 ml = 0.05 L of this prepared stock. This is an addition volume, not stock strength or final-medium concentration.

Fatty acid mixture

Dose: 0.02 L

Stock composition
Ingredient	Concentration	Notes
Isobutyric acid	0.023 L	Preparation input: 23 ml = 0.023 L. This is input volume, not a mass, stock dose or measured final-volume denominator.
DL-2-Methylbutyric acid	0.027 L	Preparation input: 27 ml = 0.027 L. This is input volume, not a mass, stock dose or measured final-volume denominator.
Valeric acid	0.027 L	Preparation input: 27 ml = 0.027 L. This is input volume, not a mass, stock dose or measured final-volume denominator.
Isovaleric acid	0.027 L	Preparation input: 27 ml = 0.027 L. This is input volume, not a mass, stock dose or measured final-volume denominator.
Distilled water	0.896 L	Preparation input: 896 ml = 0.896 L. This is input volume, not a mass, stock dose or measured final-volume denominator.

Receiving-medium addition: 20 ml = 0.02 L of this prepared stock. This is an addition volume, not stock strength or final-medium concentration.

Sodium resazurin (0.1% w/v)

Dose: 0.0005 L

Stock composition
Ingredient	Concentration	Notes
Sodium resazurin	0.1 PERCENT_W_V	Explicit source stock strength; keep separate from its receiving-medium dose. No density or final-medium dilution is inferred. Copied annotations do not establish the source's exact chemical form; withdraw them, preserve the literal source name, and archive the prior node. No replacement ontology identifier is inferred.

Receiving-medium addition: 0.5 ml = 0.0005 L of this prepared stock. This is an addition volume, not stock strength or final-medium concentration.

Target organisms

No target organisms are recorded.

Recipe variants

Name
For DSM 1093, DSM 1125, DSM 11977, DSM 11978, DSM 11995, DSM 16643
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Supplement medium after autoclaving with 1 ml/l Wolin's vitamin solution (10x, see medium 120), 0.30 g/l DL-dithiothreitol, and 0.15 g/l coenzyme M (2-mercaptoethanesulfonic acid) from anoxic stock solutions sterilized by filtration. Omit Na2S x 9 H2O and L-Cysteine HCl x H2O.
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 1093, DSM 1125, DSM 11977, DSM 11978, DSM 11995, DSM 16643: Supplement medium after autoclaving with 1 ml/l Wolin's vitamin solution (10x, see medium 120), 0.30 g/l DL-dithiothreitol, and 0.15 g/l coenzyme M (2-mercaptoethanesulfonic acid) from anoxic stock solutions sterilized by filtration. Omit Na2S x 9 H2O and L-Cysteine HCl x H2O.
  Explanation
  variants[0].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 1093, DSM 1125, DSM 11977, DSM 11978, DSM 11995, DSM 16643: Supplement medium after autoclaving with 1 ml/l Wolin's vitamin solution (10x, see medium 120), 0.30 g/l DL-dithiothreitol, and 0.15 g/l coenzyme M (2-mercaptoethanesulfonic acid) from anoxic stock solutions sterilized by filtration. Omit Na2S x 9 H2O and L-Cysteine HCl x H2O. variants[0].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 1093, DSM 1125, DSM 11977, DSM 11978, DSM 11995, DSM 16643: Supplement medium after autoclaving with 1 ml/l Wolin's vitamin solution (10x, see medium 120), 0.30 g/l DL-dithiothreitol, and 0.15 g/l coenzyme M (2-mercaptoethanesulfonic acid) from anoxic stock solutions sterilized by filtration. Omit Na2S x 9 H2O and L-Cysteine HCl x H2O. variants[0].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.
Name
For DSM 1535
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Adjust pH of complete medium to 7.6.
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 1535: Adjust pH of complete medium to 7.6.
  Explanation
  variants[1].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 1535: Adjust pH of complete medium to 7.6. variants[1].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 1535: Adjust pH of complete medium to 7.6. variants[1].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.
Name
For DSM 2030
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation.
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 2030: Adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation.
  Explanation
  variants[2].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 2030: Adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. variants[2].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 2030: Adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. variants[2].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.
Name
For DSM 3267, DSM 11074, DSM 11075
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Adjust pH of complete medium to 8.0 - 8.3.
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 3267, DSM 11074, DSM 11075: Adjust pH of complete medium to 8.0 - 8.3.
  Explanation
  variants[3].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 3267, DSM 11074, DSM 11075: Adjust pH of complete medium to 8.0 - 8.3. variants[3].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 3267, DSM 11074, DSM 11075: Adjust pH of complete medium to 8.0 - 8.3. variants[3].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.
Name
For DSM 5522
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Supplement medium with 5.0 g/l D-glucose added after autoclaving from a sterile anoxic stock solution.
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 5522: Supplement medium with 5.0 g/l D-glucose added after autoclaving from a © 2022 DSMZ - All rights reserved Page 1 of 3 119: METHANOBACTERIUM MEDIUM sterile anoxic stock solution.
  Explanation
  variants[4].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 5522: Supplement medium with 5.0 g/l D-glucose added after autoclaving from a © 2022 DSMZ - All rights reserved Page 1 of 3 119: METHANOBACTERIUM MEDIUM sterile anoxic stock solution. variants[4].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 5522: Supplement medium with 5.0 g/l D-glucose added after autoclaving from a © 2022 DSMZ - All rights reserved Page 1 of 3 119: METHANOBACTERIUM MEDIUM sterile anoxic stock solution. variants[4].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.
Name
For DSM 6216
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Increase amount of Na-acetate to 3.00 g/l.
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 6216: Increase amount of Na-acetate to 3.00 g/l.
  Explanation
  variants[5].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 6216: Increase amount of Na-acetate to 3.00 g/l. variants[5].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 6216: Increase amount of Na-acetate to 3.00 g/l. variants[5].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.
Name
For DSM 7057
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Supplement medium with 2.00 g/l Na2SO4.
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 7057: Supplement medium with 2.00 g/l Na2SO4.
  Explanation
  variants[6].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 7057: Supplement medium with 2.00 g/l Na2SO4. variants[6].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 7057: Supplement medium with 2.00 g/l Na2SO4. variants[6].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.
Name
For DSM 7095, DSM 28100
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Supplement medium with 0.3 g DL-Dithiothreitol. Omit Na2S x 9 H2O.
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 7095, DSM 28100: Supplement medium with 0.3 g DL-Dithiothreitol. Omit Na2S x 9 H2O.
  Explanation
  variants[7].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 7095, DSM 28100: Supplement medium with 0.3 g DL-Dithiothreitol. Omit Na2S x 9 H2O. variants[7].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 7095, DSM 28100: Supplement medium with 0.3 g DL-Dithiothreitol. Omit Na2S x 9 H2O. variants[7].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.
Name
For DSM 9575
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation.
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 9575: Add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation.
  Explanation
  variants[8].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 9575: Add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. variants[8].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 9575: Add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. variants[8].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.
Name
For DSM 15163
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Supplement medium with 0.3 g DL-Dithiothreitol. Omit Na2S x 9 H2O. Adjust pH of final medium to 6.0.
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 15163: Supplement medium with 0.3 g DL-Dithiothreitol. Omit Na2S x 9 H2O. Adjust pH of final medium to 6.0.
  Explanation
  variants[9].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 15163: Supplement medium with 0.3 g DL-Dithiothreitol. Omit Na2S x 9 H2O. Adjust pH of final medium to 6.0. variants[9].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 15163: Supplement medium with 0.3 g DL-Dithiothreitol. Omit Na2S x 9 H2O. Adjust pH of final medium to 6.0. variants[9].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.
Name
For DSM 16632
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310) and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of Wolin's vitamin solution (see medium 141).
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 16632: Replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310) and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of Wolin's vitamin solution (see medium 141).
  Explanation
  variants[10].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 16632: Replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310) and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of Wolin's vitamin solution (see medium 141). variants[10].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 16632: Replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310) and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of Wolin's vitamin solution (see medium 141). variants[10].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.
Name
For DSM 25824
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Supplement medium after autoclaving with 0.10 g/l 2-mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation.
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 25824: Supplement medium after autoclaving with 0.10 g/l 2-mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation.
  Explanation
  variants[11].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 25824: Supplement medium after autoclaving with 0.10 g/l 2-mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. variants[11].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 25824: Supplement medium after autoclaving with 0.10 g/l 2-mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. variants[11].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.
Name
For DSM 25939
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Adjust pH of the complete medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation.
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 25939: Adjust pH of the complete medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation.
  Explanation
  variants[12].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 25939: Adjust pH of the complete medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. variants[12].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 25939: Adjust pH of the complete medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. variants[12].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.
Name
For DSM 25945
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Adjust pH of the complete medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation.
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 25945: Adjust pH of the complete medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation.
  Explanation
  variants[13].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 25945: Adjust pH of the complete medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. variants[13].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 25945: Adjust pH of the complete medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. variants[13].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.
Name
For DSM 102889
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Adjust pH of complete medium to 8.5 - 9.0.
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 102889: Adjust pH of complete medium to 8.5 - 9.0.
  Explanation
  variants[14].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 102889: Adjust pH of complete medium to 8.5 - 9.0. variants[14].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 102889: Adjust pH of complete medium to 8.5 - 9.0. variants[14].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.
Name
For DSM 108286
Relationship
STRAIN_SPECIFIC_VARIANT
Modifications
- Adjust pH of final medium to 6.0.
Evidence
- Reference
  https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf
  Supports
  SUPPORT
  Snippet
  For DSM 108286: Adjust pH of final medium to 6.0.
  Explanation
  variants[15].name: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 108286: Adjust pH of final medium to 6.0. variants[15].relationship: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a. Additional exact excerpt: For DSM 108286: Adjust pH of final medium to 6.0. variants[15].modifications: Explicit original strain instruction, separate from the base formulation. This does not assert measured growth or add a taxon association. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T02:29:16.493797+00:00; capture SHA256=416f170d6c2424ded82767e6ae67497a313eafa144a00e140f01107a60fa8c4a.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:10.205063Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 119
2026-03-13T03:09:18.105211Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 1 duplicate ingredient(s)
2026-03-13T06:18:57.012957Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000643
2026-03-17T06:30:26.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:08.167408+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "METHANOBACTERIUM MEDIUM" to "methanobacterium_medium" for consistent matching
2026-05-20T06:01:55.056271+00:00	migrate_ph_range.py	MIGRATED_PH_RANGE	ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0}
2026-06-05T07:41:30.695344+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.655326+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-09T03:06:56.858381+00:00	codex	RESTORED_MEDIUM_119_SOURCE_FORMULATIONS_AND_STOCK_SCOPES_WITH_EVIDENCE	Restore exact-source medium-119 ingredients, liquid inputs, stock strengths/doses and preparation scopes; preserve source-specific variants, quantity limits and disagreements, and repair reciprocal family references without inferring growth.