methanoregula_peat_medium

Overview

External ontology

mediadive.medium:1280 (source link unavailable) — METHANOREGULA (PEAT) MEDIUM

Applications

• Microbial cultivation

Synonyms

• {'name': 'methanoregula_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:1280', 'original_category': 'archaea'}

pH

5.1

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1280.pdf

Composition

flowchart LR
    CultureMech_000744["methanoregula_peat_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_000744 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000744 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_KCl["KCl"]
    CultureMech_000744 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_Na_acetate["Na-acetate"]
    CultureMech_000744 --> ing3_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing3_Na_acetate -.-> CHEBI_32954
    ing4_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000744 --> ing4_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Sodium_resazurin -.-> CHEBI_8806
    ing5_Homo_PIPES["Homo-PIPES"]
    CultureMech_000744 --> ing5_Homo_PIPES
    ing6_NaOH["NaOH"]
    CultureMech_000744 --> ing6_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing6_NaOH -.-> CHEBI_32145
    ing7_Nitrilotriacetate["Nitrilotriacetate"]
    CultureMech_000744 --> ing7_Nitrilotriacetate
    CHEBI_25548["CHEBI:25548"]
    ing7_Nitrilotriacetate -.-> CHEBI_25548
    ing8_TiCl3["TiCl3"]
    CultureMech_000744 --> ing8_TiCl3
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_000744 --> ing9_Yeast_extract
    ing10_2_Mercaptoethanesulfonate["2-Mercaptoethanesulfonate"]
    CultureMech_000744 --> ing10_2_Mercaptoethanesulfonate
    CHEBI_17905["CHEBI:17905"]
    ing10_2_Mercaptoethanesulfonate -.-> CHEBI_17905
    ing11_HCl["HCl"]
    CultureMech_000744 --> ing11_HCl
    CHEBI_17883["CHEBI:17883"]
    ing11_HCl -.-> CHEBI_17883
    ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000744 --> ing12_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000744 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_ZnCl2["ZnCl2"]
    CultureMech_000744 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_H3BO3["H3BO3"]
    CultureMech_000744 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000744 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000744 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing18_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
    CultureMech_000744 --> ing18_MnSO4_x_4_H2O
    CHEBI_86358["CHEBI:86358"]
    ing18_MnSO4_x_4_H2O -.-> CHEBI_86358
    ing19_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000744 --> ing19_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing19_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing20_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000744 --> ing20_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing20_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000744 --> ing21_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing22_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_000744 --> ing22_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing22_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing23_Biotin["Biotin"]
    CultureMech_000744 --> ing23_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing23_Biotin -.-> CHEBI_15956
    ing24_Folic_acid["Folic acid"]
    CultureMech_000744 --> ing24_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing24_Folic_acid -.-> CHEBI_27470
    ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000744 --> ing25_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing26_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000744 --> ing26_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing26_Thiamine_HCl -.-> CHEBI_49105
    ing27_Riboflavin["Riboflavin"]
    CultureMech_000744 --> ing27_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing27_Riboflavin -.-> CHEBI_17015
    ing28_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000744 --> ing28_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing28_Nicotinic_acid -.-> CHEBI_15940
    ing29_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_000744 --> ing29_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing29_Calcium_D_____pantothenate -.-> CHEBI_31345
    CultureMech_000744 --> more["...3 more"]

Ingredients (33)

Preparation

1. Dissolve ingredients of solution A, adjust pH to 5.0 with 0.1 N HCl, and sparge with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense solution A under the same gas atmosphere into anoxic serum vials (20 ml medium in 100 ml bottles) and autoclave. Solutions B to F are prepared under a 100% N2 gas atmosphere and sterilized by filtration. Before inoculation, complete the medium by adding the appropriate amounts of solutions B to F to sterile solution A in the sequence indicated.
2. Adjust pH of the complete medium to 5.1 with a sterile anoxic solution of 1 N HCl. After inoculation pressurize vials to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
3. Note: Use 10-20% (v/v) as inoculum.
4. Adjust pH of buffer to 5.5.
5. First dissolve ferrous chloride in the HCl, then dilute in water and dissolve the other salts. Finally make up to 1000 ml.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

• methanoregula_medium (CultureMech:004033)
• methanoregula_peat_medium (CultureMech:000744)