Overview
- External ontology
- mediadive.medium:1280 (source link unavailable) — METHANOREGULA (PEAT) MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.1
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1280.pdf
Composition
flowchart LR
CultureMech_000744["methanoregula_peat_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_000744 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KH2PO4["KH2PO4"]
CultureMech_000744 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_KCl["KCl"]
CultureMech_000744 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_Na_acetate["Na-acetate"]
CultureMech_000744 --> ing3_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing3_Na_acetate -.-> CHEBI_32954
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_000744 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5_Homo_PIPES["Homo-PIPES"]
CultureMech_000744 --> ing5_Homo_PIPES
ing6_NaOH["NaOH"]
CultureMech_000744 --> ing6_NaOH
CHEBI_32145["CHEBI:32145"]
ing6_NaOH -.-> CHEBI_32145
ing7_Nitrilotriacetate["Nitrilotriacetate"]
CultureMech_000744 --> ing7_Nitrilotriacetate
CHEBI_25548["CHEBI:25548"]
ing7_Nitrilotriacetate -.-> CHEBI_25548
ing8_TiCl3["TiCl3"]
CultureMech_000744 --> ing8_TiCl3
ing9_Yeast_extract["Yeast extract"]
CultureMech_000744 --> ing9_Yeast_extract
ing10_2_Mercaptoethanesulfonate["2-Mercaptoethanesulfonate"]
CultureMech_000744 --> ing10_2_Mercaptoethanesulfonate
CHEBI_17905["CHEBI:17905"]
ing10_2_Mercaptoethanesulfonate -.-> CHEBI_17905
ing11_HCl["HCl"]
CultureMech_000744 --> ing11_HCl
CHEBI_17883["CHEBI:17883"]
ing11_HCl -.-> CHEBI_17883
ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_000744 --> ing12_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000744 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_ZnCl2["ZnCl2"]
CultureMech_000744 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_H3BO3["H3BO3"]
CultureMech_000744 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000744 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000744 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
CultureMech_000744 --> ing18_MnSO4_x_4_H2O
CHEBI_86358["CHEBI:86358"]
ing18_MnSO4_x_4_H2O -.-> CHEBI_86358
ing19_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000744 --> ing19_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing19_MgSO4_x_7_H2O -.-> CHEBI_31795
ing20_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000744 --> ing20_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing20_CaCl2_x_2_H2O -.-> CHEBI_86158
ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000744 --> ing21_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
ing22_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_000744 --> ing22_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing22_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing23_Biotin["Biotin"]
CultureMech_000744 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_Folic_acid["Folic acid"]
CultureMech_000744 --> ing24_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing24_Folic_acid -.-> CHEBI_27470
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000744 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Thiamine_HCl["Thiamine HCl"]
CultureMech_000744 --> ing26_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing26_Thiamine_HCl -.-> CHEBI_49105
ing27_Riboflavin["Riboflavin"]
CultureMech_000744 --> ing27_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing27_Riboflavin -.-> CHEBI_17015
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_000744 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_000744 --> ing29_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing29_Calcium_D_____pantothenate -.-> CHEBI_31345
CultureMech_000744 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.0283019 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.0146751 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.00209644 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.0178197 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000524109 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 | Homo-PIPES | 158 G_PER_L | ||
| 7 |
NaOH
HO.Na 39.997 g/mol
|
18.109432 G_PER_L |
CHEBI:32145
sodium hydroxide
|
[Merged 2 duplicates: 18.0, 0.109432] |
| 8 |
Nitrilotriacetate
C6H6NO6 188.115 g/mol
|
0.458736 G_PER_L |
CHEBI:25548
nitrilotriacetate(3-)
|
|
| 9 | TiCl3 | 6.34615 G_PER_L | ||
| 10 | Yeast extract | 20 G_PER_L | ||
| 11 |
2-Mercaptoethanesulfonate
C2H6O3S2 142.201 g/mol
|
7 G_PER_L |
CHEBI:17905
coenzyme M
|
|
| 12 |
HCl
HCl 36.461 g/mol
|
2.47525 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 13 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.32673 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.0237624 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.0742574 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.0188119 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.0237624 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0237624 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 19 |
MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
|
0.0257426 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
|
| 20 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.54455 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 21 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
2.31683 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 22 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.00891089 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 23 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
3.41584 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 25 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 27 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 28 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 30 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 31 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 32 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 33 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients of solution A, adjust pH to 5.0 with 0.1 N HCl, and sparge with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense solution A under the same gas atmosphere into anoxic serum vials (20 ml medium in 100 ml bottles) and autoclave. Solutions B to F are prepared under a 100% N2 gas atmosphere and sterilized by filtration. Before inoculation, complete the medium by adding the appropriate amounts of solutions B to F to sterile solution A in the sequence indicated.
- Adjust pH of the complete medium to 5.1 with a sterile anoxic solution of 1 N HCl. After inoculation pressurize vials to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
- Note: Use 10-20% (v/v) as inoculum.
- Adjust pH of buffer to 5.5.
- First dissolve ferrous chloride in the HCl, then dilute in water and dissolve the other salts. Finally make up to 1000 ml.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.980915Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1280 |
| 2026-02-06T05:10:39.703066Z | invalid-chebi-removal | Removed 2 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:20.963365Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:58.014748Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000744 |
| 2026-03-17T06:30:29.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.361084+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOREGULA (PEAT) MEDIUM" to "methanoregula_peat_medium" for consistent matching |
| 2026-06-05T07:41:32.164596+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.211025+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |