Overview
- External ontology
- mediadive.medium:1331 (source link unavailable) — COLUMBIA AGAR WITH 5% SHEEP BLOOD
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1331.pdf
Composition
flowchart LR
CultureMech_000788["columbia_agar_with_5_sheep_blood"]
ing0_Defibrinated_Blood["Defibrinated Blood"]
CultureMech_000788 --> ing0_Defibrinated_Blood
ing1_Bacto_peptone["Bacto peptone"]
CultureMech_000788 --> ing1_Bacto_peptone
ing2_Tryptic_Digest_of_beef_heart["Tryptic Digest of beef heart"]
CultureMech_000788 --> ing2_Tryptic_Digest_of_beef_heart
ing3_Corn_starch["Corn starch"]
CultureMech_000788 --> ing3_Corn_starch
CHEBI_28017["CHEBI:28017"]
ing3_Corn_starch -.-> CHEBI_28017
ing4_NaCl["NaCl"]
CultureMech_000788 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Agar["Agar"]
CultureMech_000788 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Casein_peptone["Casein peptone"]
CultureMech_000788 --> ing6_Casein_peptone
ing7_Soy_peptone["Soy peptone"]
CultureMech_000788 --> ing7_Soy_peptone
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Defibrinated Blood | 50 G_PER_L | ||
| 2 | Bacto peptone | 19.04762 G_PER_L | [Merged 2 duplicates: 9.52381, 9.52381] | |
| 3 | Tryptic Digest of beef heart | 2.85714 G_PER_L | ||
| 4 | Corn starch | 0.952381 G_PER_L |
CHEBI:28017
starch
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
9.7619 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 4.7619, 5.0] |
| 6 | Agar | 14.2857 G_PER_L |
CHEBI:2509
agar
|
|
| 7 | Casein peptone | 15 G_PER_L | ||
| 8 | Soy peptone | 5 G_PER_L |
Preparation
- 1. Suspend 44 g Columbia Blood Agar Base in 1l deionized water. Heat to boiling to dissolve completely.
- 2. Sterilize by autoclaving for 15 min at 121°C.
- 3. Cool to 45°C. Add 5% sterile defibrinated sheep blood and mix well.
- Rehydrate pellets of freeze-dried ampoules in liquid medium 220 (see below).
- Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid Cm131).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- columbia_agar_with_5_sheep_blood (CultureMech:000788)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.121119Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1331 |
| 2026-01-28T19:48:40.991342Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:40.744826Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:18:58.374458Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000788 |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.774389+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "COLUMBIA AGAR WITH 5% SHEEP BLOOD" to "columbia_agar_with_5_sheep_blood" for consistent matching |
| 2026-06-05T07:40:54.634978+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:28.971996+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e5d7808b77a9b559b544f1012673b62c548608930918aa5da5cd3a62e5e2b3ab |