columbia_agar_with_5_sheep_blood

Overview

External ontology

mediadive.medium:1331 (source link unavailable) — COLUMBIA AGAR WITH 5% SHEEP BLOOD

Applications

• Microbial cultivation

pH

7.3

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1331.pdf

Composition

flowchart LR
    CultureMech_000788["columbia_agar_with_5_sheep_blood"]
    ing0_Defibrinated_Blood["Defibrinated Blood"]
    CultureMech_000788 --> ing0_Defibrinated_Blood
    ing1_Bacto_peptone["Bacto peptone"]
    CultureMech_000788 --> ing1_Bacto_peptone
    ing2_Tryptic_Digest_of_beef_heart["Tryptic Digest of beef heart"]
    CultureMech_000788 --> ing2_Tryptic_Digest_of_beef_heart
    ing3_Corn_starch["Corn starch"]
    CultureMech_000788 --> ing3_Corn_starch
    CHEBI_28017["CHEBI:28017"]
    ing3_Corn_starch -.-> CHEBI_28017
    ing4_NaCl["NaCl"]
    CultureMech_000788 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Agar["Agar"]
    CultureMech_000788 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Casein_peptone["Casein peptone"]
    CultureMech_000788 --> ing6_Casein_peptone
    ing7_Soy_peptone["Soy peptone"]
    CultureMech_000788 --> ing7_Soy_peptone

Ingredients (8)

Preparation

1. 1. Suspend 44 g Columbia Blood Agar Base in 1l deionized water. Heat to boiling to dissolve completely.
2. 2. Sterilize by autoclaving for 15 min at 121°C.
3. 3. Cool to 45°C. Add 5% sterile defibrinated sheep blood and mix well.
4. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 (see below).
5. Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid Cm131).

Target organisms

No target organisms are recorded.

Provenance