1_10_pygv_medium_modified

CultureMech:000859 bacterial COMPLEX LIQUID
Original name: 1/10 PYGV MEDIUM modified

Overview

External ontology
mediadive.medium:1398 (source link unavailable) — 1/10 PYGV MEDIUM modified
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1398.pdf

Composition

flowchart LR
    CultureMech_000859["1_10_pygv_medium_modified"]
    ing0_Trypticase["Trypticase"]
    CultureMech_000859 --> ing0_Trypticase
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000859 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_000859 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_L_Cysteine_HCl["L-Cysteine HCl"]
    CultureMech_000859 --> ing3_L_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing3_L_Cysteine_HCl -.-> CHEBI_91247
    ing4_Resazurin["Resazurin"]
    CultureMech_000859 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_Cobalamine["Cobalamine"]
    CultureMech_000859 --> ing5_Cobalamine
    CHEBI_30411["CHEBI:30411"]
    ing5_Cobalamine -.-> CHEBI_30411
    ing6_K2HPO4["K2HPO4"]
    CultureMech_000859 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_KH2PO4["KH2PO4"]
    CultureMech_000859 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000859 --> ing8__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing8__NH4_2SO4 -.-> CHEBI_62946
    ing9_NaCl["NaCl"]
    CultureMech_000859 --> ing9_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing9_NaCl -.-> CHEBI_26710
    ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000859 --> ing10_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000859 --> ing11_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing11_CaCl2_x_2_H2O -.-> CHEBI_86158

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Trypticase 1 G_PER_L MICRO:0000175 (source link unavailable)
Trypticase peptone
2 Yeast extract 0.0005 G_PER_L
3 Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17234
glucose
4 L-Cysteine HCl
C3H7NO2S.HCl 157.622 g/mol
0.0003 G_PER_L CHEBI:91247
L-cysteine hydrochloride
5 Resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
6 Cobalamine 0.1 G_PER_L CHEBI:30411
cobalamin
7 K2HPO4
HO4P.2K 174.174 g/mol
6 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 KH2PO4
H2O4P.K 136.084 g/mol
6 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
9 (NH4)2SO4
2H4N.O4S 132.141 g/mol
12 G_PER_L CHEBI:62946
ammonium sulfate
10 NaCl
Cl.Na 58.443 g/mol
12 G_PER_L CHEBI:26710
sodium chloride
11 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
12 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.2 G_PER_L CHEBI:86158
calcium chloride dihydrate

Preparation

  1. pH 7.0
  2. Prepare the medium without the vitamin solution under an atmosphere of N2 and dispense into Hungate tubes. Seal under an atmosphere of nitrogen with rubber stoppers and autoclave. Aseptically add the vitamin solution from a filter sterilised stock solution to the sterile medium.
  3. SII

Solutions

100x Vitamin solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
Biotin 0.1 G_PER_L
Thiamine-HCl x 2 H2O 0.5 G_PER_L
p-Aminobenzoic acid 0.3 G_PER_L
Pyridoxine 1.5 G_PER_L
Riboflavin 0.5 G_PER_L
Folic acid 0.5 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:14.308896Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1398
2026-01-28T19:48:15.912495Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:58.971439Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000859
2026-03-17T06:29:59.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.436400+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "1/10 PYGV MEDIUM modified" to "1_10_pygv_medium_modified" for consistent matching
2026-06-05T07:40:15.399944+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.469274+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:21.499935+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 6 stock-strength component(s) out of ingredients into 1 solution(s): 6 -> '100x Vitamin solution' @ 10 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).
2026-09-22T00:03:26.686152+00:00 repair_rejected_peptone_groundings.py CORRECTED_REJECTED_PEPTONE_GROUNDING CultureMech #484. /ingredients/0/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved.
2026-09-22T00:08:22.856832+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: 1_10_pygv_medium_modified, KOMODO_1398_1_10_PYGV_medium_modified
2026-09-22T00:08:22.856856+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint ac1dbd20ce5ff9830ea45f9c3efb6788dd56dbfa0dce226c632a2dc4e09d7653