Overview
- External ontology
- komodo.medium:1398 (source link unavailable) — 1/10 PYGV medium modified
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: KOMODO ModelSEED | ID: 1398 | DSMZ Medium: 1398 (mediadive.medium:1398) | Aerobic: Yes
Composition
flowchart LR
CultureMech_004115["1_10_pygv_medium_modified"]
ing0_Trypticase["Trypticase"]
CultureMech_004115 --> ing0_Trypticase
ing1_Yeast_extract["Yeast extract"]
CultureMech_004115 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_004115 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_L_Cysteine_HCl["L-Cysteine HCl"]
CultureMech_004115 --> ing3_L_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing3_L_Cysteine_HCl -.-> CHEBI_91247
ing4_Resazurin["Resazurin"]
CultureMech_004115 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Cobalamine["Cobalamine"]
CultureMech_004115 --> ing5_Cobalamine
CHEBI_30411["CHEBI:30411"]
ing5_Cobalamine -.-> CHEBI_30411
ing6_K2HPO4["K2HPO4"]
CultureMech_004115 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_KH2PO4["KH2PO4"]
CultureMech_004115 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8__NH4_2SO4["(NH4)2SO4"]
CultureMech_004115 --> ing8__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing8__NH4_2SO4 -.-> CHEBI_62946
ing9_NaCl["NaCl"]
CultureMech_004115 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004115 --> ing10_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004115 --> ing11_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase | 1 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
|
| 2 | Yeast extract | 0.0005 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 |
L-Cysteine HCl
C3H7NO2S.HCl 157.622 g/mol
|
0.0003 G_PER_L |
CHEBI:91247
L-cysteine hydrochloride
|
|
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 | Cobalamine | 0.1 G_PER_L |
CHEBI:30411
cobalamin
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 9 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
12 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
12 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 11 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 12 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Solutions
100x Vitamin solution
Dose: 10 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 0.1 G_PER_L | |
| Thiamine-HCl x 2 H2O | 0.5 G_PER_L | |
| p-Aminobenzoic acid | 0.3 G_PER_L | |
| Pyridoxine | 1.5 G_PER_L | |
| Riboflavin | 0.5 G_PER_L | |
| Folic acid | 0.5 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1398 |
| 2026-02-03T11:19:02.435589 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1398 |
| 2026-03-13T06:19:24.968272Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004115 |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:39.803167+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "1/10 PYGV medium modified" to "1_10_pygv_medium_modified" for consistent matching |
| 2026-06-05T07:40:21.110407+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.197225+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T20:44:33.187372+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 6 stock-strength component(s) out of ingredients into 1 solution(s): 6 -> '100x Vitamin solution' @ 10 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/0/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |