Overview
- External ontology
- mediadive.medium:139 (source link unavailable) — HATHEWAYA MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'clostridium_histolyticum_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:139', 'original_category': 'bacterial'}
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium139.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_000860["hathewaya_medium"]
ing0_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
CultureMech_000860 --> ing0_Pancreatic_digest_of_casein
ing1_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
CultureMech_000860 --> ing1_Peptic_digest_of_soybean_meal
ing2_Glucose["Glucose"]
CultureMech_000860 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Sodium_chloride["Sodium chloride"]
CultureMech_000860 --> ing3_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_chloride -.-> CHEBI_26710
ing4_Dipotassium_phosphate["Dipotassium phosphate"]
CultureMech_000860 --> ing4_Dipotassium_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Dipotassium_phosphate -.-> CHEBI_131527
ing5_Agar["Agar"]
CultureMech_000860 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Proteose_peptone["Proteose peptone"]
CultureMech_000860 --> ing6_Proteose_peptone
ing7_Na2HPO4["Na2HPO4"]
CultureMech_000860 --> ing7_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing7_Na2HPO4 -.-> CHEBI_34683
ing8_KH2PO4["KH2PO4"]
CultureMech_000860 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000860 --> ing9_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
ing10_Sodium_resazurin["Sodium resazurin"]
CultureMech_000860 --> ing10_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Sodium_resazurin -.-> CHEBI_8806
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
17.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 2 |
Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
3.0 G_PER_L |
FOODON:03315720
vegetable protein, hydrolyzed
|
Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 3 |
Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 4 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 5 |
Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 6 |
Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
15.0 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 7 | Proteose peptone | 50 G_PER_L | ||
| 8 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
9 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.92 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.08 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 11 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
Preparation
- Dissolve ingredients, adjust pH to 7.2, bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- clostridium_histolyticum_medium (CultureMech:004116)
- hathewaya_medium (CultureMech:000860)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.296078Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 139 |
| 2026-03-13T06:18:58.977003Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000860 |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.442691+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HATHEWAYA MEDIUM" to "hathewaya_medium" for consistent matching |
| 2026-06-05T07:41:08.317960+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.705984+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:28.144257+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: clostridium_histolyticum_medium, hathewaya_medium |
| 2026-08-06T03:05:28.144327+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 7f553020320113c4f62fa2aff147d1c66d0470b7bf0e83af4913c4cffc21dad4 |