clostridium_histolyticum_medium

CultureMech:004116 bacterial COMPLEX LIQUID
Original name: CLOSTRIDIUM HISTOLYTICUM medium

Overview

External ontology
komodo.medium:139 (source link unavailable) — CLOSTRIDIUM HISTOLYTICUM medium
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: KOMODO ModelSEED | ID: 139 | DSMZ Medium: 139 (mediadive.medium:139) | Aerobic: No Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_004116["clostridium_histolyticum_medium"]
    ing0_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_004116 --> ing0_Pancreatic_digest_of_casein
    ing1_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_004116 --> ing1_Peptic_digest_of_soybean_meal
    ing2_Glucose["Glucose"]
    CultureMech_004116 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Sodium_chloride["Sodium chloride"]
    CultureMech_004116 --> ing3_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing3_Sodium_chloride -.-> CHEBI_26710
    ing4_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_004116 --> ing4_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing4_Dipotassium_phosphate -.-> CHEBI_131527
    ing5_Agar["Agar"]
    CultureMech_004116 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Proteose_peptone["Proteose peptone"]
    CultureMech_004116 --> ing6_Proteose_peptone
    ing7_Na2HPO4["Na2HPO4"]
    CultureMech_004116 --> ing7_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing7_Na2HPO4 -.-> CHEBI_34683
    ing8_KH2PO4["KH2PO4"]
    CultureMech_004116 --> ing8_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing8_KH2PO4 -.-> CHEBI_63036
    ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_004116 --> ing9_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing10_Sodium_resazurin["Sodium resazurin"]
    CultureMech_004116 --> ing10_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Sodium_resazurin -.-> CHEBI_8806

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
17.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
2 Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
3.0 G_PER_L FOODON:03315720
vegetable protein, hydrolyzed
 Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
3 Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:17234
glucose
 Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
4 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
5 Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
6 Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
15.0 G_PER_L CHEBI:2509
agar
 Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
7 Proteose peptone 50 G_PER_L
8 Na2HPO4
HO4P.2Na 141.958 g/mol
9 G_PER_L CHEBI:34683
disodium hydrogenphosphate
9 KH2PO4
H2O4P.K 136.084 g/mol
1.92 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
10 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.08 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
11 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 139
2026-02-03T11:19:19.074006 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 139
2026-03-13T06:19:24.973347Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:004116
2026-03-17T06:30:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:39.809045+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CLOSTRIDIUM HISTOLYTICUM medium" to "clostridium_histolyticum_medium" for consistent matching
2026-06-05T07:40:53.939541+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.700106+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.