Overview
- External ontology
- mediadive.medium:1423 (source link unavailable) — METHYLOSINUS MEDIUM MM2
- Applications
-
- Microbial cultivation
- pH
- 5.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1423.pdf
Composition
flowchart LR
CultureMech_000887["methylosinus_medium_mm2"]
ing0_KNO3["KNO3"]
CultureMech_000887 --> ing0_KNO3
CHEBI_63043["CHEBI:63043"]
ing0_KNO3 -.-> CHEBI_63043
ing1_KH2PO4["KH2PO4"]
CultureMech_000887 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000887 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000887 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_EDTA["EDTA"]
CultureMech_000887 --> ing4_EDTA
CHEBI_4735["CHEBI:4735"]
ing4_EDTA -.-> CHEBI_4735
ing5_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
CultureMech_000887 --> ing5_CuCl2_x_5_H2O
CHEBI_91245["CHEBI:91245"]
ing5_CuCl2_x_5_H2O -.-> CHEBI_91245
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000887 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000887 --> ing7_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing8_H3BO3["H3BO3"]
CultureMech_000887 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000887 --> ing9_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing9_NiCl2_x_6_H2O -.-> CHEBI_34887
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000887 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_Na2MoO4["Na2MoO4"]
CultureMech_000887 --> ing11_Na2MoO4
CHEBI_75215["CHEBI:75215"]
ing11_Na2MoO4 -.-> CHEBI_75215
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000887 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KNO3
K.NO3 101.102 g/mol
|
0.2 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.04 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.02 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.004 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
EDTA
C10H16N2O8 292.244 g/mol
|
5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 6 |
CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
|
0.1 G_PER_L |
CHEBI:91245
copper(II) chloride pentahydrate
|
|
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 8 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 10 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
Na2MoO4
MoO4.2Na 205.916 g/mol
|
0.03 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Preparation
- Adjust pH to 5.0.
- Dispense the medium into serum bottles under gassing with CH4 (20% v/v).
- Autoclave at 121 °C for 20 min.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methylosinus_medium_mm2 (CultureMech:000887)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.381946Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1423 |
| 2026-03-13T06:18:59.234871Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000887 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.726322+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHYLOSINUS MEDIUM MM2" to "methylosinus_medium_mm2" for consistent matching |
| 2026-06-05T07:41:33.397712+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.576794+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:07.661934+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:45.087291+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a1cf64b476a96ef56914dca6b34a75b17b7aa9c9b8d89957d583e6b7ab527fae |