methylosinus_medium_mm2

CultureMech:000887 bacterial DEFINED LIQUID
Original name: METHYLOSINUS MEDIUM MM2

Overview

External ontology
mediadive.medium:1423 (source link unavailable) — METHYLOSINUS MEDIUM MM2
Applications
  • Microbial cultivation
pH
5.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1423.pdf

Composition

flowchart LR
    CultureMech_000887["methylosinus_medium_mm2"]
    ing0_KNO3["KNO3"]
    CultureMech_000887 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000887 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000887 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000887 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_EDTA["EDTA"]
    CultureMech_000887 --> ing4_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing4_EDTA -.-> CHEBI_4735
    ing5_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
    CultureMech_000887 --> ing5_CuCl2_x_5_H2O
    CHEBI_91245["CHEBI:91245"]
    ing5_CuCl2_x_5_H2O -.-> CHEBI_91245
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000887 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000887 --> ing7_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing8_H3BO3["H3BO3"]
    CultureMech_000887 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000887 --> ing9_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing9_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000887 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_Na2MoO4["Na2MoO4"]
    CultureMech_000887 --> ing11_Na2MoO4
    CHEBI_75215["CHEBI:75215"]
    ing11_Na2MoO4 -.-> CHEBI_75215
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000887 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 KNO3
K.NO3 101.102 g/mol
0.2 G_PER_L CHEBI:63043
potassium nitrate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.04 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.02 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.004 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
6 CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
0.1 G_PER_L CHEBI:91245
copper(II) chloride pentahydrate
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
8 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
9 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
10 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 Na2MoO4
MoO4.2Na 205.916 g/mol
0.03 G_PER_L CHEBI:75215
sodium molybdate (anhydrous)
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate

Preparation

  1. Adjust pH to 5.0.
  2. Dispense the medium into serum bottles under gassing with CH4 (20% v/v).
  3. Autoclave at 121 °C for 20 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.381946Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1423
2026-03-13T06:18:59.234871Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000887
2026-03-17T06:29:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.726322+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOSINUS MEDIUM MM2" to "methylosinus_medium_mm2" for consistent matching
2026-06-05T07:41:33.397712+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.576794+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:07.661934+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.