Overview
- External ontology
- mediadive.medium:143 (source link unavailable) — CLOSTRIDIUM SP. LA1 MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'clostridium_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:143', 'original_category': 'bacterial'}
- pH range
- {'min': 6.8, 'max': 7.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium143.pdf
Composition
flowchart LR
CultureMech_000902["clostridium_sp_la1_medium"]
ing0_Crotonic_acid["Crotonic acid"]
CultureMech_000902 --> ing0_Crotonic_acid
CHEBI_41131["CHEBI:41131"]
ing0_Crotonic_acid -.-> CHEBI_41131
ing1_NaOH["NaOH"]
CultureMech_000902 --> ing1_NaOH
CHEBI_32145["CHEBI:32145"]
ing1_NaOH -.-> CHEBI_32145
ing2__NH4_2HPO4["(NH4)2HPO4"]
CultureMech_000902 --> ing2__NH4_2HPO4
CHEBI_63051["CHEBI:63051"]
ing2__NH4_2HPO4 -.-> CHEBI_63051
ing3_K2HPO4["K2HPO4"]
CultureMech_000902 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000902 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000902 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_NH4Cl["NH4Cl"]
CultureMech_000902 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000902 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_MnSO4_x_2_H2O["MnSO4 x 2 H2O"]
CultureMech_000902 --> ing8_MnSO4_x_2_H2O
CHEBI_86356["CHEBI:86356"]
ing8_MnSO4_x_2_H2O -.-> CHEBI_86356
ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000902 --> ing9_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
ing10__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_000902 --> ing10__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing10__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing11_Biotin["Biotin"]
CultureMech_000902 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_000902 --> ing12_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing12_p_Aminobenzoic_acid -.-> CHEBI_30753
ing13_Tryptone["Tryptone"]
CultureMech_000902 --> ing13_Tryptone
ing14_Yeast_extract["Yeast extract"]
CultureMech_000902 --> ing14_Yeast_extract
ing15_Sodium_resazurin["Sodium resazurin"]
CultureMech_000902 --> ing15_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing15_Sodium_resazurin -.-> CHEBI_8806
ing16_K2CO3["K2CO3"]
CultureMech_000902 --> ing16_K2CO3
CHEBI_131526["CHEBI:131526"]
ing16_K2CO3 -.-> CHEBI_131526
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Crotonic acid
C4H6O2 86.09 g/mol
|
6 G_PER_L |
CHEBI:41131
crotonic acid
|
|
| 2 |
NaOH
HO.Na 39.997 g/mol
|
0.3 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 3 |
(NH4)2HPO4
2H4N.HO4P 132.056 g/mol
|
0.15 G_PER_L |
CHEBI:63051
diammonium hydrogen phosphate
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.03 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.04 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.05 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.0006 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
MnSO4 x 2 H2O
2H2O.Mn.O4S 187.031 g/mol
|
0.0004 G_PER_L |
CHEBI:86356
manganese(II) sulfate dihydrate
|
|
| 10 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.0004 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 11 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
0.01 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
4e-05 G_PER_L |
CHEBI:15956
biotin
|
|
| 13 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.0008 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 14 | Tryptone | 1 G_PER_L | ||
| 15 | Yeast extract | 1 G_PER_L | ||
| 16 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 17 |
K2CO3
CO3.2K 138.204 g/mol
|
4 G_PER_L |
CHEBI:131526
potassium carbonate
|
Preparation
- Dissolve ingredients (except potassium carbonate) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. An anoxic stock solution of the potassium carbonate is autoclaved separately under 80% N2 and 20% CO2 gas mixture and thereafter added to the sterile medium. Adjust pH of the complete medium to 6.8 - 7.0. Prior to inoculation add 10 - 20 mg/l sodium dithionite from a 5% (w/v) stock solution that has been freshly prepared under 100% N2 gas and sterilized by filtration to reduce the medium.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- clostridium_medium (CultureMech:004158)
- clostridium_sp_la1_medium (CultureMech:000902)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.327455Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 143 |
| 2026-03-13T06:18:59.380875Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000902 |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.891192+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CLOSTRIDIUM SP. LA1 MEDIUM" to "clostridium_sp_la1_medium" for consistent matching |
| 2026-05-20T06:01:12.055369+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0} |
| 2026-06-05T07:40:54.200687+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.645625+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:54.801914+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:28.392187+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: clostridium_medium, clostridium_sp_la1_medium |
| 2026-08-06T03:05:28.392241+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 54b6136ac7a9e4a81f3f7e6f3dd492f206e2f98e822258f35af329bb7f12b072 |