clostridium_sp_la1_medium

CultureMech:000902 bacterial COMPLEX LIQUID
Original name: CLOSTRIDIUM SP. LA1 MEDIUM

Overview

External ontology
mediadive.medium:143 (source link unavailable) — CLOSTRIDIUM SP. LA1 MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.8, 'max': 7.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium143.pdf

Composition

flowchart LR
    CultureMech_000902["clostridium_sp_la1_medium"]
    ing0_Crotonic_acid["Crotonic acid"]
    CultureMech_000902 --> ing0_Crotonic_acid
    CHEBI_41131["CHEBI:41131"]
    ing0_Crotonic_acid -.-> CHEBI_41131
    ing1_NaOH["NaOH"]
    CultureMech_000902 --> ing1_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing1_NaOH -.-> CHEBI_32145
    ing2__NH4_2HPO4["(NH4)2HPO4"]
    CultureMech_000902 --> ing2__NH4_2HPO4
    CHEBI_63051["CHEBI:63051"]
    ing2__NH4_2HPO4 -.-> CHEBI_63051
    ing3_K2HPO4["K2HPO4"]
    CultureMech_000902 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000902 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000902 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_NH4Cl["NH4Cl"]
    CultureMech_000902 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000902 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_MnSO4_x_2_H2O["MnSO4 x 2 H2O"]
    CultureMech_000902 --> ing8_MnSO4_x_2_H2O
    CHEBI_86356["CHEBI:86356"]
    ing8_MnSO4_x_2_H2O -.-> CHEBI_86356
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000902 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_000902 --> ing10__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing10__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing11_Biotin["Biotin"]
    CultureMech_000902 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000902 --> ing12_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing12_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing13_Tryptone["Tryptone"]
    CultureMech_000902 --> ing13_Tryptone
    ing14_Yeast_extract["Yeast extract"]
    CultureMech_000902 --> ing14_Yeast_extract
    ing15_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000902 --> ing15_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing15_Sodium_resazurin -.-> CHEBI_8806
    ing16_K2CO3["K2CO3"]
    CultureMech_000902 --> ing16_K2CO3
    CHEBI_131526["CHEBI:131526"]
    ing16_K2CO3 -.-> CHEBI_131526

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Crotonic acid
C4H6O2 86.09 g/mol
6 G_PER_L CHEBI:41131
crotonic acid
2 NaOH
HO.Na 39.997 g/mol
0.3 G_PER_L CHEBI:32145
sodium hydroxide
3 (NH4)2HPO4
2H4N.HO4P 132.056 g/mol
0.15 G_PER_L CHEBI:63051
diammonium hydrogen phosphate
4 K2HPO4
HO4P.2K 174.174 g/mol
0.1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.03 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.04 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 NH4Cl
Cl.H4N 53.492 g/mol
0.05 G_PER_L CHEBI:31206
ammonium chloride
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.0006 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
9 MnSO4 x 2 H2O
2H2O.Mn.O4S 187.031 g/mol
0.0004 G_PER_L CHEBI:86356
manganese(II) sulfate dihydrate
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.0004 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
11 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.01 G_PER_L CHEBI:91249
ammonium molybdate
12 Biotin
C10H16N2O3S 244.316 g/mol
4e-05 G_PER_L CHEBI:15956
biotin
13 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.0008 G_PER_L CHEBI:30753
4-aminobenzoic acid
14 Tryptone 1 G_PER_L
15 Yeast extract 1 G_PER_L
16 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
17 K2CO3
CO3.2K 138.204 g/mol
4 G_PER_L CHEBI:131526
potassium carbonate

Preparation

  1. Dissolve ingredients (except potassium carbonate) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. An anoxic stock solution of the potassium carbonate is autoclaved separately under 80% N2 and 20% CO2 gas mixture and thereafter added to the sterile medium. Adjust pH of the complete medium to 6.8 - 7.0. Prior to inoculation add 10 - 20 mg/l sodium dithionite from a 5% (w/v) stock solution that has been freshly prepared under 100% N2 gas and sterilized by filtration to reduce the medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.327455Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 143
2026-03-13T06:18:59.380875Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000902
2026-03-17T06:30:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.891192+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CLOSTRIDIUM SP. LA1 MEDIUM" to "clostridium_sp_la1_medium" for consistent matching
2026-05-20T06:01:12.055369+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0}
2026-06-05T07:40:54.200687+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.645625+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:54.801914+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.