Overview
- External ontology
- mediadive.medium:1483 (source link unavailable) — HALARCHAEUM MEDIUM RS94
- Applications
-
- Microbial cultivation
- pH
- 7.1
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1483.pdf
Composition
flowchart LR
CultureMech_000951["halarchaeum_medium_rs94"]
ing0_NaCl["NaCl"]
CultureMech_000951 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000951 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_K2SO4["K2SO4"]
CultureMech_000951 --> ing2_K2SO4
CHEBI_32036["CHEBI:32036"]
ing2_K2SO4 -.-> CHEBI_32036
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000951 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
CultureMech_000951 --> ing4_NaH2PO4_x_2_H2O
CHEBI_37585["CHEBI:37585"]
ing4_NaH2PO4_x_2_H2O -.-> CHEBI_37585
ing5_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_000951 --> ing5_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing5_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing6_Na_pyruvate["Na pyruvate"]
CultureMech_000951 --> ing6_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing6_Na_pyruvate -.-> CHEBI_50144
ing7_Na_acetate["Na acetate"]
CultureMech_000951 --> ing7_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing7_Na_acetate -.-> CHEBI_32954
ing8_Na_malate["Na malate"]
CultureMech_000951 --> ing8_Na_malate
CHEBI_91260["CHEBI:91260"]
ing8_Na_malate -.-> CHEBI_91260
ing9_Casein_hydrolysate["Casein hydrolysate"]
CultureMech_000951 --> ing9_Casein_hydrolysate
ing10_Yeast_extract["Yeast extract"]
CultureMech_000951 --> ing10_Yeast_extract
ing11_Tryptone["Tryptone"]
CultureMech_000951 --> ing11_Tryptone
ing12_Glucose["Glucose"]
CultureMech_000951 --> ing12_Glucose
CHEBI_17234["CHEBI:17234"]
ing12_Glucose -.-> CHEBI_17234
ing13_Agar["Agar"]
CultureMech_000951 --> ing13_Agar
CHEBI_2509["CHEBI:2509"]
ing13_Agar -.-> CHEBI_2509
ing14_EDTA["EDTA"]
CultureMech_000951 --> ing14_EDTA
CHEBI_4735["CHEBI:4735"]
ing14_EDTA -.-> CHEBI_4735
ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000951 --> ing15_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000951 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000951 --> ing17_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
ing18_H3BO3["H3BO3"]
CultureMech_000951 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000951 --> ing19_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000951 --> ing20_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000951 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000951 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
25 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
K2SO4
2K.O4S 174.259 g/mol
|
5 G_PER_L |
CHEBI:32036
potassium sulfate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
NaH2PO4 x 2 H2O
H2O4P.Na 119.976 g/mol
|
0.2 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 6 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
0.4 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 7 |
Na pyruvate
C3H3O3.Na 110.044 g/mol
|
2 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 8 |
Na acetate
C2H3O2.Na 82.034 g/mol
|
0.5 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 9 | Na malate | 0.5 G_PER_L |
CHEBI:91260
disodium malate
|
|
| 10 | Casein hydrolysate | 2 G_PER_L | ||
| 11 | Yeast extract | 1 G_PER_L | ||
| 12 | Tryptone | 0.5 G_PER_L | ||
| 13 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 14 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
|
| 15 |
EDTA
C10H16N2O8 292.244 g/mol
|
5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 16 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 18 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 21 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.03 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust to pH 7.1
- pH 3.0-4.0
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- halarchaeum_medium_rs94 (CultureMech:000951)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.566555Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1483 |
| 2026-03-13T06:18:59.839135Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000951 |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.392657+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALARCHAEUM MEDIUM RS94" to "halarchaeum_medium_rs94" for consistent matching |
| 2026-06-05T07:41:07.110397+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.301208+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:28.614784+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 18b55a44d27ab42969729c98b087bfa86e5d1cc2b6c5517181f64002f105eb18 |