halarchaeum_medium_rs94

CultureMech:000951 archaea COMPLEX SOLID_AGAR
Original name: HALARCHAEUM MEDIUM RS94

Overview

External ontology
mediadive.medium:1483 (source link unavailable) — HALARCHAEUM MEDIUM RS94
Applications
  • Microbial cultivation
pH
7.1
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1483.pdf

Composition

flowchart LR
    CultureMech_000951["halarchaeum_medium_rs94"]
    ing0_NaCl["NaCl"]
    CultureMech_000951 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000951 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_K2SO4["K2SO4"]
    CultureMech_000951 --> ing2_K2SO4
    CHEBI_32036["CHEBI:32036"]
    ing2_K2SO4 -.-> CHEBI_32036
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000951 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
    CultureMech_000951 --> ing4_NaH2PO4_x_2_H2O
    CHEBI_37585["CHEBI:37585"]
    ing4_NaH2PO4_x_2_H2O -.-> CHEBI_37585
    ing5_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_000951 --> ing5_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing5_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing6_Na_pyruvate["Na pyruvate"]
    CultureMech_000951 --> ing6_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing6_Na_pyruvate -.-> CHEBI_50144
    ing7_Na_acetate["Na acetate"]
    CultureMech_000951 --> ing7_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing7_Na_acetate -.-> CHEBI_32954
    ing8_Na_malate["Na malate"]
    CultureMech_000951 --> ing8_Na_malate
    CHEBI_91260["CHEBI:91260"]
    ing8_Na_malate -.-> CHEBI_91260
    ing9_Casein_hydrolysate["Casein hydrolysate"]
    CultureMech_000951 --> ing9_Casein_hydrolysate
    ing10_Yeast_extract["Yeast extract"]
    CultureMech_000951 --> ing10_Yeast_extract
    ing11_Tryptone["Tryptone"]
    CultureMech_000951 --> ing11_Tryptone
    ing12_Glucose["Glucose"]
    CultureMech_000951 --> ing12_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing12_Glucose -.-> CHEBI_17234
    ing13_Agar["Agar"]
    CultureMech_000951 --> ing13_Agar
    CHEBI_2509["CHEBI:2509"]
    ing13_Agar -.-> CHEBI_2509
    ing14_EDTA["EDTA"]
    CultureMech_000951 --> ing14_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing14_EDTA -.-> CHEBI_4735
    ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000951 --> ing15_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000951 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000951 --> ing17_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing18_H3BO3["H3BO3"]
    CultureMech_000951 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000951 --> ing19_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000951 --> ing20_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000951 --> ing21_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000951 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
25 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 K2SO4
2K.O4S 174.259 g/mol
5 G_PER_L CHEBI:32036
potassium sulfate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 NaH2PO4 x 2 H2O
H2O4P.Na 119.976 g/mol
0.2 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
6 Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
0.4 G_PER_L CHEBI:34683
disodium hydrogenphosphate
7 Na pyruvate
C3H3O3.Na 110.044 g/mol
2 G_PER_L CHEBI:50144
sodium pyruvate
8 Na acetate
C2H3O2.Na 82.034 g/mol
0.5 G_PER_L CHEBI:32954
sodium acetate
9 Na malate 0.5 G_PER_L CHEBI:91260
disodium malate
10 Casein hydrolysate 2 G_PER_L
11 Yeast extract 1 G_PER_L
12 Tryptone 0.5 G_PER_L
13 Glucose
C6H12O6 180.156 g/mol
0.5 G_PER_L CHEBI:17234
glucose
14 Agar 20 G_PER_L CHEBI:2509
agar
15 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
16 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
19 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
20 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
21 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.03 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
22 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust to pH 7.1
  2. pH 3.0-4.0

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:14.566555Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1483
2026-03-13T06:18:59.839135Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000951
2026-03-17T06:30:27.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.392657+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALARCHAEUM MEDIUM RS94" to "halarchaeum_medium_rs94" for consistent matching
2026-06-05T07:41:07.110397+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.301208+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.